Bioinstrumentation for Crop and Animal Genome Analysis

9318032翼本提案的总体目标是获得高通量自动化系统的植物和动物基因组分析的仪器资源。 这项技术将用于遗传图谱的构建、多样性分析、谱系构建、复杂性状分析、基于图谱的基因克隆和基因组数据库开发。 所要求的系统将支持七个主要用户研究小组和另外8个研究实验室的多学科研究。 自动化系统所需的仪器资源包括：用于样品制备和储存的大容量冷冻干燥器，可以以96孔格式使用的荧光计，其输出通过计算机连接用于自动样品稀释，用于自动液体处理和光度测量任务的机器人，用于扩增DNA标记的专用PCR仪器，用于分析扩增的多态性DNA和用于获取微卫星、cDNA和其他标记序列的自动DNA测序仪，用于数据捕获和存储的凝胶图像，以及用于数据分析和基因组信息学的UNIX工作站。 仪器资源将被安置在2,500平方英尺的作物基因组分析实验室，该实验室位于德克萨斯州A M大学新的作物生物技术中心。 作物生物技术中心将提供仪器资源的维护和操作。 首席研究员Rod Wing博士将监督自动基因组分析系统的运行。 基因组分析系统的日常操作和调度将由作物生物技术中心提供的科学家进行。

9318032 Wing The overall objective of this proposal is to acquire instrument resources for a high throughput automated system for plant and animal genome analysis. This enabling technology will be used for genetic map construction, diversity analysis, pedigree construction, analysis of complex traits, map-based gene cloning and genome database development. The requested system will support the multidisciplinary research of seven major user research groups and 8 additional research laboratories. The instrument resources required for the automated system include; a large capacity freeze dryer for sample preparation and storage, a fluorimeter which can be used in a 96 well format with output connected via computer for automated sample dilution, a robot for automated liquid handing and photometric measurement tasks, dedicated PCR instruments for amplification of DNA markers, an automated DNA sequencer for analysis of amplified polymorphic DNA and for acquisition of microsatellite, cDNA and other marker sequences, a gel image for data capture and storage, and a UNIX Workstation for data analysis and genome informatics. The instrument resources will be housed in the 2,500 square foot Laboratory for Crop Genome Analysis which is located in a new Crop Biotechnology Center at Texas A&M University. The Crop Biotechnology Center will provide for the maintenance and operation of the instrument resource. The principal investigator, Dr. Rod Wing, will oversee the operation of the automated genome analysis system. Day-to- day operation and scheduling of the genome analysis system will be carried out by a staff scientist provided through the Crop Biotechnology Center.