Acquisition of Major Multiuser Research Instrumentation for the Study of Plant, Fungal and Insect Genomics

这项提议的主要目标是获得主要仪器，用于复杂植物、真菌和昆虫基因组分析的研究和培训。该仪器包括：5台自动DNA测序仪，1台用于准备DNA测序模板的机器人，9台用于处理序列和物理图谱数据的计算机工作站，用于开发用于文库筛选和植物育种应用的数千个DNA样本高通量筛选的半自动程序的机器人系统，用于维护大插入B AC文库的12台超低冰柜，6个杂交孵化器的库，以及用于快速筛选和分析BAC文库的多功能成像系统。所要求的仪器将与现有的机器人设备以及正在进行的研究、服务和教学计划相结合，以在克莱姆森大学(CU)建立一个最先进的基因组中心。这个新的CU基因组中心的功能是：1)构建和维护高质量的BAC文库；2)生产和分发高密度杂交过滤器和DNA池，用于文库筛选和BAC文库和克隆的分发；3)农业重要基因的定位克隆；4)利用BAC克隆对整个染色体和目标基因组区域进行物理定位；5)重要BAC重叠群和基因组的基因组测序；6)应用开发；以及7)本科生、研究生和博士后培训。通过为这项提案提供资金，将大大加强三项主要活动。首先，核磁共振资金将确保CU基因组中心将能够作为公共资源，用于构建、维护和分发重要植物、真菌和昆虫基因组的大插入BAC文库。这种集中的资源将使研究界能够方便地使用用于位置克隆、物理测绘和基因组测序的主要工具。其次，核磁共振资金将使我们能够执行旨在物理测绘和测序基因组的特定基因组区域的试点项目，这些区域是密集研究的目标。这些项目的成功完成将使加州大学基因组中心能够有效地竞争联邦政府的大量支持，对拟南芥、水稻和粗糙脉孢菌等基因组的主要部分进行测序。最后，核磁共振资金将大大支持和加强基因组学本科生、研究生和博士后的培训。通过使用最先进的仪器，CU-Genome中心将吸引高素质的学生和学者，推动几个生命科学项目进入全国排名，如遗传学跨学科项目。如上所述，CU基因组中心将是一个国际公认的设施，反过来将产生为整个农业基因组界服务的世界级科学。

The primary objective of this proposal is to acquire major instrumentation for research and training in the analyses of complex plant, fungal and insect genomes. The instrumentation includes: 5 automated DNA sequencers, a robot for preparing DNA sequencing templates, 9 computer workstations for processing sequence and physical mapping data, a robotics system for the development of semi-automated procedures for high through-put screening of thousands of DNA samples for library screening and plant breeding applications, 12 ultra-low freezers for maintenance of large insert B AC libraries, a bank of 6 hybridization incubators and a versatile imaging system for rapid screening and analysis of BAC libraries. The requested instrumentation will be integrated with existing robotics equipment, and ongoing research, service and teaching programs to establish a state-of-the-art Genome Center at Clemson University (CU). The functions of this new CU Genome Center are: 1 ) construction and maintenance of high quality BAC libraries; 2) production and distribution of high-density hybridization filters and DNA pools for library screening and distribution of BAC libraries and clones; 3) positional cloning of agriculturally important genes; 4) physical mapping of whole chromosomes and targeted genomic regions with BAC clones; 5) genome sequencing of important BAC contigs and genomes; 6) application development; and 7) undergraduate, graduate and postdoctoral training. There are three primary activities that would be greatly enhanced by funding this proposal. First, MRI funding would ensure that the CU Genome Center will be capable of serving as a public resource for the construction, maintenance and distribution of large insert BAC libraries of important plant, fungal and insect genomes. Such a centralized resource would provide the research community convenient access to a major tool that is used for positional cloning, physical mapping and genome sequencing. Secondly, MRI funding would allow us to perf orm pilot projects aimed at physical mapping and sequencing of specific genomic regions of genomes that are being targeted for intensive investigation. Successful completion of these projects will put the CU Genome Center in a position to compete effectively for substantial federal support to sequence major portions of genomes such as Arabidopsis, Rice and Neurospora crassa. Lastly, MRI funding will substantially support and enhance undergraduate, graduate, and postdoctoral training in genomics. With access to state-of-the-art instrumentation, the CU-Genome Center will attract students and scholars of high caliber propelling several life sciences programs to national ranking, such as the interdisciplinary program in Genetics. The CU Genome Center, as envisioned above, will be an internationally recognized facility that in turn will produce world class science serving the agricultural genome community at large.