Sequencing Rice Chromosome 3

项目摘要 0241181该奖项支持CCW水稻基因组测序联盟[克莱姆森大学基因组研究所（CUGI），冷泉港实验室（CSHL）和华盛顿大学。基因组测序中心（WUGSC）]继续他们的水稻基因组测序项目，该项目于1999年10月获得资助。该项目的最初目标是作为国际水稻基因组测序计划（IRGSP）的一部分，对10号和3号染色体短臂上的约20 Mb水稻基因组DNA进行生产测序、完成和注释。第10号染色体基本完成，正在编写联合手稿。3号染色体正在进行中。该奖项与基因组研究所（TIGR）合作，具体目标是对水稻3号染色体进行完整测序、完成、注释和发表。具体目标是：1）在2002年12月之前完成水稻3号染色体的第二阶段草案（目前的资金已经完成了约75%）; 2）与TIGR合作，在2003年9月之前完成水稻3号染色体，以支持IRGSP将完成的水稻基因组存入GenBank的最终目标。 研究计划概要如下：A）44个~ 143 kb日本晴BAC克隆（CUGI/Monsanto）锚定到3号染色体短臂的BAC克隆将被验证插入完整性和染色体定位B）将进行44个BAC克隆的文库开发和生产测序（10 X）C）将完成44个生产测序的BAC D）完成技术开发，旨在建立强大的化学和协议，可用于财团完成水稻基因组的困难区域。44个完成的BAC将进行第一阶段注释。所有序列数据将根据“百慕大”政策公开发布。

PROJECT ABSTRACT 0241181This award support the CCW Rice Genome Sequencing Consortium [Clemson Univ.Genomics Institute (CUGI), Cold Spring Harbor Laboratory (CSHL) and WashingtonUniv. Genome Sequencing Center (WUGSC)] to continue their rice genome sequencing project that was funded in October 1999. The original goal of the project was to productionsequence, finish and annotate approximately 20 Mb of rice genomic DNA on the shortarms of chromosomes 10 and 3 as part of the International Rice Genome SequencingProject (IRGSP). Chromosome 10 is essentially finished and a joint manuscript is in preparation. Chromosome 3 is in progress. In collaboration with the Institute for Genomic Research (TIGR), the specific objectives of this award is to completely sequence, finish, annotate and publish rice chromosomes 3. The specific goals are: 1) to generate a Phase II draft of rice chromosome 3 (~75% done already with current funding) by December 2002; and 2) completely finish rice chromosome 3 by September 2003, in collaboration with TIGR, in support of the ultimate IRGSP objective of depositing a finished rice genome in GenBank. An outline of research plan is: A) Forty-four ~143kb Nipponbare BAC clones (CUGI/Monsanto) anchored to the shortarm of chromosome 3 will be validated for insert integrity and chromosomal locationB) Library development and production sequencing (10X) of the 44 BAC clones will beperformed C) 44 production sequenced BACs will be finished D) finishing technology development aimed at establishing robust chemistries and protocols that can be used by the consortium to finish difficult regions of the rice genome.E) 44 finished BACs will be phase I annotated All sequence data will be publicly released according to the "Bermuda" policies.