The Role of Cauliflower in Specifying Floral Meristem Identity in Arabidopsis

花椰菜在指定拟南芥花分生组织特性中的作用

• 批准号: 9404037
• 负责人: Martin Yanofsky
• 金额: $ 36万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-01 至 1997-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9404037&HistoricalAwards=false
• 关键词: Role; Cauliflower; Specifying; Floral; Meristem

After floral induction, the first step in the development of flowers is the generation of floral meristems. At least three genes, CAULIFLOWER, APETALA1, and LEAFY, are necessary for the specification of floral meristem identity in Arabidopsis. This investigator has recently isolated the CAULIFLOWER and APETALA1 genes. This proposal describes a combined genetic and molecular approach for determining how CAULIFLOWER interacts with other genes to promote flowering. The investigator proposes to isolate and characterize an allelic series of cal mutants and analyze the resulting phenotypes by scanning electron microscopy. The transcriptional regulation of CAULIFLOWER will be investigated by determining the spatial and temporal pattern of expression in a variety of mutant backgrounds. Additional studies will determine if the CAULIFLOWER protein interacts directly with APETALA1, and will identify the DNA sequences to which CAULIFLOWER specifically binds. Further studies will investigate the cis- and trans-acting factors needed to promote floral meristem-specific expression of CAL, and will determine the molecular basis for the functional redundancy of CAL and AP1. These studies will be integrated into efforts to understand the role of APETALA1 and should lead to significant contributions towards understanding the genetic and molecular control of floral meristem specification. The cauliflower phenotype in Arabidopsis is strikingly similar to the anomalous inflorescence structures produced in the dinner table cauliflower, Brassica oleracea. Since Arabidopsis and Brassica are closely related plant species, and since the phenotypes of these mutants is similar, the investigator will use the cloned CAL and AP1 genes to investigate the molecular basis of the cauliflower phenotype. Finally, since the cauliflower phenotype has been described in plants that are distantly related to Arabidopsis, and since the CAULIFLOWER gene has not been isolated from any other plant s pecies, the characterization of this gene should yield significant insights into its role in diverse plant systems.

花诱导后，花发育的第一步是花分生组织的产生。 至少三个基因，CAULIFLOWER、APETALA1 和 LEAFY，对于拟南芥花分生组织特性的规范是必需的。 该研究人员最近分离出了 CAULIFLOWER 和 APETALA1 基因。 该提案描述了一种组合的遗传和分子方法，用于确定花椰菜如何与其他基因相互作用以促进开花。 研究人员建议分离和表征一系列 cal 突变体的等位基因，并通过扫描电子显微镜分析由此产生的表型。 将通过确定各种突变背景中表达的空间和时间模式来研究 CAULIFLOWER 的转录调控。 其他研究将确定 CAULIFLOWER 蛋白是否直接与 APETALA1 相互作用，并将鉴定 CAULIFLOWER 特异性结合的 DNA 序列。 进一步的研究将调查促进 CAL 花分生组织特异性表达所需的顺式和反式作用因子，并将确定 CAL 和 AP1 功能冗余的分子基础。 这些研究将被纳入了解 APETALA1 作用的努力中，并应为了解花分生组织规范的遗传和分子控制做出重大贡献。 拟南芥中的花椰菜表型与餐桌花椰菜（Brassica oleracea）中产生的异常花序结构惊人地相似。 由于拟南芥和芸苔属是密切相关的植物物种，并且由于这些突变体的表型相似，因此研究人员将利用克隆的CAL和AP1基因来研究花椰菜表型的分子基础。 最后，由于花椰菜表型已在与拟南芥有远缘关系的植物中被描述，并且由于尚未从任何其他植物物种中分离出花椰菜基因，因此对该基因的表征应该能够对其在不同植物系统中的作用产生重要的见解。