Flower Development in Arabidopsis Thaliana

拟南芥的花发育

• 批准号: 9018749
• 负责人: Martin Yanofsky
• 金额: $ 35.09万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-15 至 1996-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018749&HistoricalAwards=false
• 关键词: Flower; Development; Arabidopsis; Thaliana

Flower development remains one of the most interesting and complex processes unique to plants, and yet little is known about the molecular mechanisms involved. Dr. Yanofsky has cloned one of the genes, Agamous (AG), that plays a key role in determining the correct pattern of floral organs in Arabidopsis thaliana. Mutations in the AG gene result in a "double flower" phenotype. Similar phenotypes have been described in other plants for as much as two thousand years, making this the oldest recognized flower abnormality. Dr. Yanofsky's goal over the next three to five years is to begin to dissect the complex regulatory network that allows the expression of the AG gene to be controlled such that expression occurs in a precise spacial and temporal manner. This will be achieved through a detailed characterization of the cis acting elements involved in regulation the expression of AG, and by identifying the sequences within the AG promoter to which trans acting regulatory factors bind. In addition, since the data suggest that the Agamous protein is a sequence specific DNA binding protein that regulates genes involved in stamen and carpel formation, he will directly test the ability of AGamous to recognize specific DNA sequences. In addition, by manipulating the expression of Agamous in transgenic plants, he will directly test predictions of a model for flower development that has thus far been based entirely on genetic data.

花的发育仍然是最有趣和最重要的研究之一。 植物特有的复杂过程，但人们对它知之甚少。 所涉及的分子机制。雅诺夫斯基博士克隆了 Agamous（AG）基因在决定 拟南芥花器官的正确模式。 AG基因突变导致“双花”表型。 类似的表型在其他植物中也有描述， 大约有两千年的历史，这是世界上最古老的 花畸形雅诺夫斯基博士未来三年的目标是 我们需要开始用五年时间来剖析复杂的监管网络 这使得AG基因的表达受到控制， 这种表达以精确的时空方式发生。 这将通过详细描述 参与AG表达调控的顺式作用元件， 并通过鉴定AG启动子内的序列， 反式作用调节因子结合。此外，由于数据 表明Agamous蛋白是一种序列特异性DNA 一种结合蛋白，调节与雄蕊和 心皮的形成，他将直接测试AGAMOS的能力， 识别特定的DNA序列。此外，通过操纵 Agamous在转基因植物中的表达，他将直接 测试花发育模型的预测， 这完全是基于基因数据。