Reactive Membranes Containing Catalytic Antibodies

9406289伦斯勒理工学院乔治·贝尔福摘要这项研究的首要目标是开发一种简单、廉价和通用的方法来将亲和力、活性或催化基团固定到合成膜上。在这种情况下，这项工作将涉及构建和在大肠杆菌中表达独特的融合蛋白基因，该融合蛋白由纤维素结合结构域和催化抗体生物合成酶部分(CBDE2-43c9)组成。纤维素结合域允许附着在纤维素膜上，而亲和基团或催化基团则为特定的应用定制膜反应器。膜反应器具有连续操作、强化传质和便于产物分离等优点，因此具有很大的吸引力。这些因素有助于使化学和生物技术行业的加工更经济。

9406289 Rensselaer Polytechnic Institute Georges Belfort ABSTRACT A foremost aim of this research will be the development of a simple, inexpensive and generic approach to immobilize affinity, reactive, or catalytic groups to synthetic membranes. In this case, the work will involve construction and expression in E. coli of a gene for unique fusion proteins consisting of a cellulose binding domain region and a catalytic antibody-biosynthetic enzyme portion (CBDE2-43C9). The cellulose binding domain permits attachment to cellulosic membranes while the affinity or catalytic group tailors the membrane reactor for specific applications. Membrane reactors are attractive because they can allow continuous operation, enhance mass transfer, and facilitate product isolation. These factors help to make processing more economical in the chemical and biotechnology industries.