Reactive Membranes Containing Catalytic Antibodies

含有催化抗体的反应膜

• 批准号: 9406289
• 负责人: Georges Belfort
• 金额: $ 3.76万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9406289&HistoricalAwards=false
• 关键词: Reactive; Membranes; Containing; Catalytic; Antibodies

9406289 Rensselaer Polytechnic Institute Georges Belfort ABSTRACT A foremost aim of this research will be the development of a simple, inexpensive and generic approach to immobilize affinity, reactive, or catalytic groups to synthetic membranes. In this case, the work will involve construction and expression in E. coli of a gene for unique fusion proteins consisting of a cellulose binding domain region and a catalytic antibody-biosynthetic enzyme portion (CBDE2-43C9). The cellulose binding domain permits attachment to cellulosic membranes while the affinity or catalytic group tailors the membrane reactor for specific applications. Membrane reactors are attractive because they can allow continuous operation, enhance mass transfer, and facilitate product isolation. These factors help to make processing more economical in the chemical and biotechnology industries.

9406289伦斯勒理工学院Georges贝尔福特 摘要 这项研究的首要目标将是开发一种简单，廉价和通用的方法来将亲合性，反应性或催化基团引入合成膜。 在这种情况下，工作将涉及在E.在大肠杆菌中表达由纤维素结合结构域区域和催化抗体生物合成酶部分组成的独特融合蛋白的基因（CBDE 2 - 43 C9）。 纤维素结合结构域允许附着于纤维素膜，而亲和或催化基团针对特定应用定制膜反应器。 膜反应器是有吸引力的，因为它们可以允许连续操作，增强传质，并促进产物分离。 这些因素有助于使化学和生物技术行业的加工更加经济。