Reactive Membranes Containing Catalytic Antibodies

摘要本研究的首要目标是开发一种简单、廉价和通用的方法来固定亲和、反应或催化基团到合成膜上。在这种情况下，这项工作将涉及在大肠杆菌中构建和表达一种独特的融合蛋白基因，该融合蛋白由纤维素结合区域和催化抗体-生物合成酶部分（CBDE2-43C9）组成。纤维素结合结构域允许附着在纤维素膜上，而亲和基团或催化基团则为特定应用定制膜反应器。膜反应器是有吸引力的，因为它们可以允许连续操作，加强传质，并促进产品分离。这些因素有助于使化学和生物技术工业的加工更加经济。

9406289 Rensselaer Polytechnic Institute Georges Belfort ABSTRACT A foremost aim of this research will be the development of a simple, inexpensive and generic approach to immobilize affinity, reactive, or catalytic groups to synthetic membranes. In this case, the work will involve construction and expression in E. coli of a gene for unique fusion proteins consisting of a cellulose binding domain region and a catalytic antibody-biosynthetic enzyme portion (CBDE2-43C9). The cellulose binding domain permits attachment to cellulosic membranes while the affinity or catalytic group tailors the membrane reactor for specific applications. Membrane reactors are attractive because they can allow continuous operation, enhance mass transfer, and facilitate product isolation. These factors help to make processing more economical in the chemical and biotechnology industries.