Acquisition of an Electrospray Mass Spectrometer

购买电喷雾质谱仪

• 批准号: 9408252
• 负责人: Gleb Mamantov
• 金额: $ 23.2万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-15 至 1997-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9408252&HistoricalAwards=false
• 关键词: Acquisition; Electrospray; Mass; Spectrometer

The University of Tennessee seeks support to expand the Chemistry Mass Spectrometry (MS) Center by adding a triple quadrupole electrospray (ES) liquid chromatography (LC)/MS system. ES is needed for expanded applications, particularly those involving characterization of biochemical systems. The instrumentation will also be used for fundamental studies of solvation and other chemical factors affecting ES sensitivity; and for investigation of the mechanism of soluble polymer size-selective capillary electrophoresis. Since ES spectra are characterized by multiple charging, the proposed 4,000 mass-to-charge (m/z) range corresponds to a practical mass range well in excess of 105. This constitutes an order-of-magnitude increase relative to current nominal capabilities, and a two order of magnitude practical improvement, in light of the special strengths (including sub-femtomole sensitivity) of ES. The system will accommodate a wide range of chromatographic solvents and flow rates, facilitating work in microscale and conventional analytical separations. The tandem MS capabilities of the instrument will provide structural insight across the full range of analyte compounds, even for individual components of unresolved or partially resolved mixtures.

田纳西大学寻求支持，通过增加三重四极杆电喷雾（ES）液相色谱（LC）/MS系统来扩大化学质谱（MS）中心。ES是需要扩大应用，特别是那些涉及生化系统的表征。该仪器也将用于溶剂化和其他化学因素影响ES灵敏度的基础研究;和可溶性聚合物尺寸选择性毛细管电泳的机制的调查。 由于ES光谱的特征在于多次充电，因此拟定的4，000质荷比（m/z）范围对应于远超过105的实际质量范围。这构成了一个数量级的增加，相对于目前的标称能力，和两个数量级的实际改进，鉴于特殊的实力（包括次毫微微摩尔灵敏度）的ES。该系统将适应广泛的色谱溶剂和流速，促进在微型和传统的分析分离工作。该仪器的串联MS功能将提供对整个分析物化合物范围的结构洞察，即使是未解析或部分解析混合物的单个组分。