RESEARCH INITIATION AWARD: Quantitative Freezing of Biological Tissue

这项提议要求支持开发一种新的实验技术，利用差示扫描量热仪(DSC)来获得冷冻过程中肝组织的动态和定量数据。这项研究将通过获得冷冻过程中已知细胞类型的生物物理参数来验证和表征DSC技术；通过监测冻融循环和降温速度的影响来研究模拟冷冻外科；并利用定量数据来构建组织冻结的热力学预测模型。

This proposal requested support to develop a new experimental technique utilizing a differential scanning calorimeter (DSC) to obtain dynamic and quantitative data of liver tissue during freezing. The reserch will validate and characterize the DSC technique by obtaining biophysical parameters of a known cell type during the freezing process; investigate a simulated cryosurgery by monitoring the effects of the freeze thaw-cycle and cooling rate; and utilize the quantative data to construct a thermodynamic predictive model of tissue freezing.