Acquisition of an EPR Spectrometer

犹他州立大学化学与生物化学系向美国国家科学基金会申请配套资金，用于购买一台电子顺磁共振光谱仪。EPR是一种基本的光谱技术，在化学和生物化学领域都有广泛的应用。犹他州立大学购买的EPR光谱仪将填补我们目前仪器设备的空白。一个新的EPR光谱仪将成为几个化学和生物化学研究项目和课程的核心。该大学目前开设的课程中使用EPR光谱仪的课程有：物理化学，高级合成，仪器分析实验室以及物理生物化学和磁共振光谱学。每门课程都将指导学生EPR光谱的基本原理以及与课程大纲相关的应用，并提供额外的“动手”操作仪器。仪器操作包括：样品制备、数据收集和数据处理。收集的数据类型（即有机自由基或过渡金属配合物）将取决于课程目标和水平。EPR光谱学也将成为整个大学和该地区研究项目的核心要素。犹他州立大学分子生物学和生物学的教员的研究项目将是新的EPR光谱仪的次要用户。此外，杨百翰大学生物化学系的一名教员和俄勒冈州立大学生物化学系的一名教员将是定期使用者。犹他州立大学化学与生物化学系确定了EPR光谱仪的七个主要用户和三个次要用户。在这些研究项目中，研究生和博士后研究助理将接受培训，记录和处理他们的数据。提案中描述的具体研究项目如下：a.阐明金属酶氮酶中MgATP水解与电子转移耦合的机制。b.红红螺旋藻一氧化碳脱氢酶镍铁硫中心的表征。c.溶蛋白气单胞菌双核水解氨基肽酶功能的EPR研究。d.光诱导抑制光合作用的机制及其与含锰水氧化配合物光组装的关系。e.铁离子在铁离子上的配位对称性和结合位点的定位。f.真菌过氧化物酶催化自由基介导的氧化和还原反应。自由基阳离子的氢原子转移反应。

The Department of Chemistry and Biochemistry at Utah State University requests matching funds from NSF to purchase an Electron Paramagnetic Resonance (EPR) Spectrometer. EPR is a fundamental spectroscopic technique with applications that span both chemistry and biochemistry. The acquisition of an EPR spectrometer by Utah State University would fill a void in our current instrumentation facilities. A new EPR spectrometer would become the centerpiece for several Chemistry and Biochemistry research programs and courses. The current courses offered by the university that would utilize an EPR spectrometer in their curricula are: Physical Chemistry, Advanced Synthesis, and Instrumental Analysis laboratories as well as Physical Biochemistry and Magnetic Resonance Spectroscopy. Each of these courses would instruct students in the fundamental principles of EPR spectroscopy as well as the applications relevant to the course outline, with additional "hands-on" operation of the instrument. Instrument operation would include: sample preparation, data collection, and data processing. The type of data collected (i.e. organic free radicals or transition metal complexes) would depend on the course objectives and level. EPR spectroscopy would also form a central element in research projects across the University and the region. Research projects of faculty members in Molecular Biology and Biology at Utah State University would be minor users of a new EPR spectrometer. In addition, a faculty member in the Biochemistry Department at Brigham Young University and a faculty member in the Biochemistry Department at Oregon State University would be periodic users. Seven major users and three minor users of an EPR spectrometer have been identified in the Department of Chemistry and Biochemistry at Utah State University. In each of these research projects, graduate students and postdoctoral research associates will be trained to record and process their data. The specific re search projects described within the proposal are listed below: a. Elucidating the Mechanism of Coupling MgATP Hydrolysis to Electron Transfer in the Metalloenzyme Nitrogenase. b. Characterization of the Nickel-Iron Sulfur Center of Carbon Monoxide Dehydrogenase from Rhodospirillum rubrum. c. EPR Investigations into the Function of the Dinuclear Hydrolytic Aminopeptidase from Aeromonas proteolytica. d. Mechanisms of the Light-Induced Inhibition of Photosynthesis and its Relation to Photoassembly of the Mn- Containing Water-Oxidizing Complex. e. Elucidation of the Coordination Symmetry and Location of the Binding Sites for Iron on Erionite. f. Free Radical Mediated Oxidative and Reductive Reactions Catalyzed by Fungal Peroxidases. g. Hydrogen Atom Transfer Reactions of Radical Cations.