SGER: Determination of Toxol Levels Using Tubulin Binding Assays

9415395穆尔昌达尼这个项目的总体目标是开发两种新的测定技术，用于紫杉醇及其活性类似物的定量测定。这些技术基于紫杉醇的能力：(A)与微管蛋白结合；(B)促进微管蛋白聚合。紫杉醇与微管蛋白结合的能力将被用来建立竞争分析，而它促进微管蛋白聚合的能力将被用来测定红豆杉提取物和血清样本中的紫杉醇及其活性类似物。***

9415395 Mulchandani The overall objective of this project is to develop two new assay techniques for the quantitative determination of taxol and its active analogs. The techniques are based on the ability of taxol to: (a) bind to tubulin; and (b) promote tubulin polymerization to microtubules. The ability of taxol to bind to tubulin is to be used to develop a competition assay, while its ability to promote tubulin polymerization is to be used to measure taxol and its active analogs in extracts of yew trees and serum samples. ***