SGER: Determination of Toxol Levels Using Tubulin Binding Assays

SGER：使用微管蛋白结合测定测定毒素水平

• 批准号: 9415395
• 负责人: Ashok Mulchandani
• 金额: $ 5万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9415395&HistoricalAwards=false
• 关键词: SGER; Determination; Toxol; Levels; Using

9415395 Mulchandani The overall objective of this project is to develop two new assay techniques for the quantitative determination of taxol and its active analogs. The techniques are based on the ability of taxol to: (a) bind to tubulin; and (b) promote tubulin polymerization to microtubules. The ability of taxol to bind to tubulin is to be used to develop a competition assay, while its ability to promote tubulin polymerization is to be used to measure taxol and its active analogs in extracts of yew trees and serum samples. ***

本项目的总体目标是建立两种新的定量测定紫杉醇及其活性类似物的方法。这些技术是基于紫杉醇的能力：(a)与微管蛋白结合；(b)促进微管蛋白聚合成微管。紫杉醇与微管蛋白结合的能力将用于开发竞争分析，而其促进微管蛋白聚合的能力将用于测量紫杉树提取物和血清样品中的紫杉醇及其活性类似物。＊＊＊