Development of Integrated Amperometric Enzyme Sensors for Glutamine and Glutamic Acid Monitoring in Mammalian Cell Cultures and Tissue

开发用于哺乳动物细胞培养物和组织中谷氨酰胺和谷氨酸监测的集成电流酶传感器

• 批准号: 9309741
• 负责人: Ashok Mulchandani
• 金额: $ 10万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 1996-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9309741&HistoricalAwards=false
• 关键词: Development; Integrated; Amperometric; Enzyme; Sensors

This project involves the development of a new integrated molecular assembly approach for construction of amperometric enzyme sensors. This approach will use chemical modification techniques to incorporate components of an amperometric enzyme sensor. The enzyme will be immobilized covalently at a chemically derivitized carbon electrode surface through a hydrophilic tether employing an avidin-biotin linkage to immobilize a large quantity of active enzyme far from the surface and out of the electical double-layer. The electron transfer mediator and the permselective membrane will be incorporated at the electrode surface in a one step operation by depositing a ferrocene conjugated polymer film electrochemically from an electrochemical bath of ferrocene conjugated monomers.

该项目涉及 发展新的综合 分子组装方法 安培酶的构建 传感器. 这种方法将使用 化学改性技术， 结合了 电流型酶传感器 的 酶将被固定化 以化学方式共价连接 衍生化碳电极表面 通过一个亲水性的系链 使用抗生物素蛋白-生物素连接 把大量的 远离表面的活性酶 在选举之外， 双层的 电子转移 介体和选择性渗透 膜将被纳入在 一步完成电极表面 沉积二茂铁操作 共轭聚合物膜 从电化学 二茂铁电化学浴 共轭单体。