Development of Integrated Amperometric Enzyme Sensors for Glutamine and Glutamic Acid Monitoring in Mammalian Cell Cultures and Tissue

本项目涉及开发一种新的集成分子组装方法，用于构建安培酶传感器。这种方法将使用化学修饰技术来整合安培酶传感器的组件。通过亲水性系链将酶共价固定在化学衍生物化的碳电极表面，采用亲水性系链采用亲和素-生物素连接来固定大量的活性酶远离表面而出电双层。通过电化学沉积二茂铁共轭聚合物薄膜，电子传递介质和过选择膜将在一步操作中被纳入电极表面二茂铁共轭单体的电化学浴。

This project involves the development of a new integrated molecular assembly approach for construction of amperometric enzyme sensors. This approach will use chemical modification techniques to incorporate components of an amperometric enzyme sensor. The enzyme will be immobilized covalently at a chemically derivitized carbon electrode surface through a hydrophilic tether employing an avidin-biotin linkage to immobilize a large quantity of active enzyme far from the surface and out of the electical double-layer. The electron transfer mediator and the permselective membrane will be incorporated at the electrode surface in a one step operation by depositing a ferrocene conjugated polymer film electrochemically from an electrochemical bath of ferrocene conjugated monomers.