Development of Integrated Amperometric Enzyme Sensors for Glutamine and Glutamic Acid Monitoring in Mammalian Cell Cultures and Tissue

该项目涉及开发一种用于构建安培酶传感器的新的集成分子组装方法。这种方法将使用化学修饰技术来整合安培酶传感器的组件。该酶将通过亲水系绳以共价方式固定在化学衍生的碳电极表面利用亲和素-生物素键将大量活性酶固定在远离表面和远离电子的地方双层的。电子转移介体和渗透选择膜将通过沉积二茂铁一步操作被结合在电极表面从二茂铁共轭单体的电化学浴中电化学形成共轭聚合物膜。

This project involves the development of a new integrated molecular assembly approach for construction of amperometric enzyme sensors. This approach will use chemical modification techniques to incorporate components of an amperometric enzyme sensor. The enzyme will be immobilized covalently at a chemically derivitized carbon electrode surface through a hydrophilic tether employing an avidin-biotin linkage to immobilize a large quantity of active enzyme far from the surface and out of the electical double-layer. The electron transfer mediator and the permselective membrane will be incorporated at the electrode surface in a one step operation by depositing a ferrocene conjugated polymer film electrochemically from an electrochemical bath of ferrocene conjugated monomers.