Molecular Regulation of Transcription Initiation

转录起始的分子调控

• 批准号: 9419784
• 负责人: William Reznikoff
• 金额: $ 35.66万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-01-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9419784&HistoricalAwards=false
• 关键词: Molecular; Regulation; Transcription; Initiation

9419784 Reznikoff This research uses Escherichia coli sigma 70 RNA polymerase and lactose (lac) operon promoter model system to study positive gene regulation by catabolite activator protein CAP. CAP is thought to activate transcription by direct interaction with RNA polymerase. One consequence of this interaction may be to stimulate an interaction between RNA polymerase and specific upstream DNA sequences. The role of candidate (-40 region) DNA sequences in the CAP activation of the lac promoter will be investigated through chemical interference and site-specific mutation studies. In addition, the possible limiting effect of these upstream sequences on lacP expression in the absence of CAP will be examined. %%% The first event in gene expression is the initiation of RNA synthesis or transcription. This multistep process involves the interaction of the enzyme RNA polymerase with specific DNA sequences called promoters. The promoter DNA sequence determines the start site and frequency of unregulated RNA synthesis. This research will investigate in detail how the bacterial regulatory protein CAP facilitates the specific interaction of RNA polymerase with the bacterial promoter lac to activate transcription of the lac genes. This CAP-lac system is a general model for understanding transcriptional activator proteins, which have been known to regulate gene expression for over 25 years. ***

9419784 Reznikoff 这项研究使用大肠杆菌 sigma 70 RNA 聚合酶和乳糖 (lac) 操纵子启动子模型系统来研究分解代谢激活蛋白 CAP 的正向基因调控。 CAP被认为通过与RNA聚合酶直接相互作用来激活转录。 这种相互作用的结果之一可能是刺激 RNA 聚合酶和特定上游 DNA 序列之间的相互作用。 将通过化学干扰和位点特异性突变研究来研究候选（-40 区域）DNA 序列在 lac 启动子的 CAP 激活中的作用。 此外，将检查在不存在 CAP 的情况下这些上游序列对 lacP 表达的可能限制作用。 %%% 基因表达的第一个事件是 RNA 合成或转录的起始。 这个多步骤过程涉及 RNA 聚合酶与称为启动子的特定 DNA 序列的相互作用。 启动子 DNA 序列决定不受调控的 RNA 合成的起始位点和频率。 本研究将详细研究细菌调节蛋白CAP如何促进RNA聚合酶与细菌启动子lac的特异性相互作用，从而激活lac基因的转录。 该 CAP-lac 系统是了解转录激活蛋白的通用模型，25 年来人们都知道转录激活蛋白可调节基因表达。 ***