Application of X-ray Absorption Spectroscopy to Study Structure in Chemical and Biological Systems

应用 X 射线吸收光谱研究化学和生物系统的结构

• 批准号: 9423181
• 负责人: Keith Hodgson
• 金额: $ 33.75万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-15 至 1999-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9423181&HistoricalAwards=false
• 关键词: Application; ray; Absorption; Spectroscopy; Study

In this project jointly funded by Experimental Physical Chemistry and Molecular and Cellular Biosciences, Keith Hodgson of Stanford University will use x-ray absorption spectroscopies to investigate the structure and function of metal ion sites in chemical and biological systems. Studies include the effect of metal oxidation state on structure and reactivity in proteins such as quinol oxidase, the mechanism of ligand binding in nitrogenase, the structural characterization of intermediates in the methane monooxygenase enzyme reaction cycle, and the elucidation of the structures of inorganic cluster complexes and bridged metal assemblies. Theoretical treatments to extend multiple scattering analysis for chemical systems will also be developed and applied to these systems. Metal sites play a key role in the specific function of biological molecules. Iron, an important component of hemoglobin, is also present in nitrogenase, the molecule which converts atmospheric nitrogen to ammonia, one of nature's fertilizers. This research will determine the structures, reactivities, and bonding of metal sites in a variety of biologically important systems. This information will be valuable in the effort to produce enzymes with chemical characteristics suited to particular needs such as the environmentally benign production of fertilizer.

在这个由实验物理化学和 分子和细胞生物科学，斯坦福大学的基思霍奇森 将使用X射线吸收光谱来研究其结构， 金属离子在化学和生物系统中的作用。 研究 包括金属氧化态对结构和反应性的影响 在蛋白质如醌醇氧化酶中， 固氮酶中间体的结构表征 甲烷单加氧酶反应循环，并阐明了 无机簇合物和桥联金属组装体的结构。 扩展多重散射分析的理论处理 还将开发化学系统并将其应用于这些系统。 金属位点在生物的特定功能中起着关键作用， 分子。 铁是血红蛋白的重要组成部分， 在固氮酶中，将大气中的氮转化为 氨，一种天然肥料。 这项研究将确定 结构，反应性，以及各种金属位点的键合 重要的生物系统。 这些信息将是有价值的， 努力生产具有适合于 特别需要，如环境友好型生产 肥料