Teacher Enhancement in Molecular Biology and Recombinant DNA Technology

由美国国家科学基金会支持的分子生物学和重组DNA技术教师提升研讨会旨在为高中生物教师提供分子生物学和重组DNA技术最新进展所需的基础知识和熟练程度。该项目的目标是改善生物学的教学，吸引更多的学生从事生物医学科学的职业，并使俄亥俄州的高中毕业生“懂DNA”。这个暑期工作坊应该包括两周的密集讲座（主题包括DNA的结构和功能，原核和真核基因的基因组大小和特性，基因克隆和重组DNA技术的原理和应用），讨论，特别研讨会（主题包括致癌基因和职业，人类基因组计划，进化和植物生物技术），俄亥俄州生物技术公司的实地考察，课程计划的制定，以及涉及简单重组DNA技术的动手实验室实验（大肠杆菌转化，质粒DNA分离，重组DNA的限制性酶切和凝胶电泳分析）。每次讲习班将选出20名教师。通过扩大外联工作，至少有一半的教师将从俄亥俄州的市中心高中积极招聘。每位参加活动的教师将获得津贴、交通费、住宿费、餐费、教科书、附件、录像带、俄亥俄州资助机构和研究资源实验室名单、电泳凝胶盒和电源、各种实验用品。教学小组将包括PI、一名教员助理、其他迈阿密教员、研究生助教、高中硕士教师、课程和评估顾问。为了增加项目的影响，PI和教员助理将进行区域和年度总结后续活动。参与教师所在学校和学区的二线教师将被纳入后续活动。作为这个项目的结果，在当前的资助期结束时，俄亥俄州100多所高中的10,000多名学生将受益。

9454355 Bhattacharjee The Teacher Enhancement workshop in molecular biology and recombinant DNA technology supported by the NSF was initiated to provide high school biology teachers with needed basic knowledge and proficiency in recent advances of molecular biology and recombinant DNA technology. The objectives of the project are to improve the teaching of biology, attract more students to careers in biomedical sciences, and make Ohio high school graduates "DNA literate." This summer workshop should involve two weeks of intensive lectures (topics include structure and function of DNA, genome size and propoerties of prokaryotic and eukaryotic genes, and the principles and applications of gene cloning and recombinant DNA technology), discussions, special seminars (topics include oncogene and career, human genome project, evolution and plant biotechnology), field trip to an Ohio biotechnology company, lesson plan development, and hands-on laboratory experiments involving simple recombinant DNA techniques (E. coli transformation, plasmid DNA Isolation, restriction digestion and gel electrophoretic analysis of recombinant DNA). Twenty teachers would be selected for each workshop. Through expanded outreach efforts, at least half of the teachers will be actively recruited from the inner-city high schools of Ohio. Each participating teacher will be provided with a stipend, travel, room and meal expenses as well as a textbook, accessories, video tapes, a list of granting agencies and research resource laboratories in Ohio, an electrophoretic gel box and power supply, and various laboratory supplies. The instructional team would include the PI, one faculty associate, other Miami faculty, graduate teaching assistants, high school master teachers, curriculum and assessment consultants. In order to increase the impact of the project, the PI and the faculty associate would conduct regional and annual summative follow-up activities. Second-tier teachers from the sch ools and districts of the participating teachers will be included in the follow-up activities. As a result of this project, at the end of the current grant period more than 10,000 students in more than 100 high schools in Ohio woul be benefitted.