Teacher Enhancement in Molecular Biology and Recombinant DNA Technology

9454355 Bhattacharjee是由国家科学基金会资助的分子生物学和重组DNA技术教师强化工作坊，旨在为高中生物教师提供分子生物学和重组DNA技术最新进展所需的基本知识和熟练程度。该项目的目标是改善生物学的教学，吸引更多的学生投身生物医学科学的职业生涯，并使俄亥俄州的高中毕业生“懂DNA”。这个暑期工作坊将包括两周的密集讲座(主题包括DNA的结构和功能、原核和真核基因的基因组大小和特性，以及基因克隆和重组DNA技术的原理和应用)、讨论、专题研讨会(主题包括癌基因和职业、人类基因组计划、进化和植物生物技术)、对俄亥俄州一家生物技术公司的实地考察、教案制定和简单重组DNA技术的动手实验室实验(大肠杆菌转化、质DNA分离、限制性内切酶和凝胶电泳分析)。每个讲习班将选出20名教师。通过扩大外展工作，至少一半的教师将从俄亥俄州市中心的高中积极招聘。每名参与的教师将获得津贴、旅费、食宿费用以及一本教科书、配件、录像带、俄亥俄州资助机构和研究资源实验室的名单、一个凝胶电泳盒和电源，以及各种实验室用品。指导团队将包括PI、一名教员助理、其他迈阿密教员、研究生助教、高中硕士教师、课程和评估顾问。为了增加该项目的影响，国际和平研究所和教员助理将开展区域和年度总结性后续活动。后续活动将包括来自学校和参与教师所在地区的二线教师。作为这一项目的结果，在当前的资助期结束时，俄亥俄州100多所高中的1万多名学生将受益。

9454355 Bhattacharjee The Teacher Enhancement workshop in molecular biology and recombinant DNA technology supported by the NSF was initiated to provide high school biology teachers with needed basic knowledge and proficiency in recent advances of molecular biology and recombinant DNA technology. The objectives of the project are to improve the teaching of biology, attract more students to careers in biomedical sciences, and make Ohio high school graduates "DNA literate." This summer workshop should involve two weeks of intensive lectures (topics include structure and function of DNA, genome size and propoerties of prokaryotic and eukaryotic genes, and the principles and applications of gene cloning and recombinant DNA technology), discussions, special seminars (topics include oncogene and career, human genome project, evolution and plant biotechnology), field trip to an Ohio biotechnology company, lesson plan development, and hands-on laboratory experiments involving simple recombinant DNA techniques (E. coli transformation, plasmid DNA Isolation, restriction digestion and gel electrophoretic analysis of recombinant DNA). Twenty teachers would be selected for each workshop. Through expanded outreach efforts, at least half of the teachers will be actively recruited from the inner-city high schools of Ohio. Each participating teacher will be provided with a stipend, travel, room and meal expenses as well as a textbook, accessories, video tapes, a list of granting agencies and research resource laboratories in Ohio, an electrophoretic gel box and power supply, and various laboratory supplies. The instructional team would include the PI, one faculty associate, other Miami faculty, graduate teaching assistants, high school master teachers, curriculum and assessment consultants. In order to increase the impact of the project, the PI and the faculty associate would conduct regional and annual summative follow-up activities. Second-tier teachers from the sch ools and districts of the participating teachers will be included in the follow-up activities. As a result of this project, at the end of the current grant period more than 10,000 students in more than 100 high schools in Ohio woul be benefitted.