Molecular Structure and Function of the Arabidopsis RPM1 Disease Resistance Gene

对导致植物抗病性的分子事件的详细了解具有实际重要性和基本兴趣。该建议采用互补的遗传学和细胞生物学方法来剖析来自模式植物拟南芥的一个称为RPM 1的特定抗性基因的结构和功能。持续的分析将确定RPM 1依赖性抗病性表达所需的新基因。研究的四个目标领域是：1。RPM 1蛋白的结构-功能分析，2.其亚细胞定位和拓扑结构; 3.与其相互作用的其他植物蛋白的数量和性质; 4.其功能所需的其他基因座的遗传鉴定。 长期以来，人们已经认识到，植物含有基因，这些基因的功能是由感染病原体的特定分离物中的特定和相应基因的存在触发的。这种“基因对基因”的相互作用负责激活一系列生物化学和细胞事件，从而导致抗病状态。此外，初始识别事件可以诱导更持久的系统性和病原体非特异性免疫，称为系统获得性抗性。近年来，一些植物抗病基因的克隆以及相应的病原基因的克隆，预示着对植物抗病性的分子和细胞反应的理解将有重大突破。一个这样的植物基因，RPM 1，从模式植物，拟南芥，将在分子和细胞水平上进行分析。将分析基因中编码的蛋白质的结构-功能、亚细胞定位和拓扑结构以及与其相互作用的其他植物蛋白质的数量和性质。将寻找其功能所需的其他遗传位点。 ***

9505678 Dangl A detailed understanding of the molecular events leading to plant disease resistance is of both practical importance and basic interest. This proposal takes complementary genetic and cell biological approaches to dissect the structure and function of a particular resistance gene, called RPM1, from the model plant Arabidopsis. Continuing analysis will identify new genes required for the expression of RPM1-dependent disease resistance. The four target areas of research are: 1. structure-function analysis of the RPM1 protein, 2. its subcellular localization and topology; 3. the number and nature of other plant proteins with which it interacts and ; 4. genetic identification of other loci required for its function. %%% It has long been recognized that plants contain genes whose function is triggered by the presence of specific and corresponding genes in particular isolates of infecting pathogens. This "gene for gene" interaction is responsible for activation of the array of biochemical and cellular events leading to a disease resistant state. In addition, the initial recognition event can induce a longer lasting, systemic and pathogen non-specific immunity" termed systemic acquired resistance. The recent cloning of several plant disease resistance genes, and the corresponding pathogen genes which trigger their action, presages fundamental breakthroughs in understanding the molecular and cellular responses of plant disease resistance. One such plant gene, RPM1, from the model plant, Arabidopsis, will be analyzed at the molecular and cellular level. The protein encoded in the gene will be analyzed for its structure-function, its subcellular localization and topology and the number and nature of other plant proteins with which it interacts. Other genetic loci required for its function will be sought. ***