Structure and Assembly of Helical Plant Viruses

9506204 Stubbs将使用本实验室已用于烟草花叶病毒(烟草花叶病毒)组的几种病毒的纤维衍射法，详细确定一些螺旋植物病毒的结构。被研究的病毒包括3种烟草病毒，即齿舌环斑病毒(ORSV)、麦草花叶病毒(RMV)和大麻花叶病毒(SHMV)，以及1种病毒--土传小麦花叶病毒(SBWMV)。这三种烟草病毒的结构将通过分子置换，利用TMV的已知结构来确定。SBWMV的结构将用本实验室发展的多维同晶取代法确定。所有结构都将使用分子动力学方法进行精炼，就像本实验室采用的用于光纤衍射数据的方法一样。这些精化方法还将进一步发展。烟草病毒组是在分子水平上已确定其三维结构的唯一杆状病毒组。待确定的结构将对理解该组的分子特征(包括亚组之间的关系)具有重要意义，并将促进对病毒组装和解体、病毒与宿主的相互作用和特异性、载体传播以及结构与RNA病毒快速进化之间的关系的理解。许多杆状植物病毒的结构将使用本实验室中已用于几种烟草病毒的纤维衍射法进行详细的分子结构测定。拟研究的病毒包括齿舌环斑病毒(ORSV)、黑麦草花叶病毒(RMV)和黑麻花叶病毒(SHMV)以及FuroVirus土传小麦花叶病毒(SBWMV)。烟草病毒组是在分子水平上已确定其三维结构的唯一杆状病毒组。待确定的结构对于了解病毒群的分子特征，包括亚群之间的关系将是重要的，并将促进对病毒组装和拆解、病毒与宿主的相互作用和特异性、病毒传播以及结构与RNA病毒特征快速进化之间的关系的理解。***

9506204 Stubbs The structures of a number of helical plant viruses will be determined in molecular detail, using the fiber diffraction methods already used in this laboratory for several viruses of the tobamovirus (tobacco mosaic virus, TMV) group. The viruses to be studied include three tobamoviruses, odontoglossum ringspot virus (ORSV), ribgrass mosaic virus (RMV) and sunn-hemp mosaic virus (SHMV), and one furovirus, soil-borne wheat mosaic virus (SBWMV). Structures of the three tobamoviruses will be determined by molecular replacement, using the known structure of TMV. The structure of SBWMV will be determined using the methods of multi- dimensional isomorphous replacement developed in this laboratory. All structures will be refined using molecular dynamics methods, as adapted in this laboratory for use with fiber diffraction data. These refinement methods will be developed further. The tobamovirus group is the only rod-shaped virus group for which three-dimensional structures have been determined at the molecular level. The structures to be determined will be important in understanding the molecular characteristics of the group, including the relationship between subgroups, and will advance understanding of viral assembly and disassembly, viral-host interactions and specificity, and vector transmission, as well as the relationship between structure and the rapid evolution of RNA viruses. %%% The structures of a number of rod-shaped plant viruses will be determined in molecular detail, using the fiber diffraction methods already used in this laboratory for several viruses of the tobamovirus. The viruses to be studied include three tobamoviruses, odontoglossum ringspot virus (ORSV), ribgrass mosaic virus (RMV) and sunn-hemp mosaic virus (SHMV), and the furovirus soil-borne wheat mosaic virus (SBWMV). The tobamovirus group is the only rod- shaped virus group for which three-dimensional structures have been determined at the molecular level. The structu res to be determined will be important in understanding the molecular characteristics of the group, including the relationship between subgroups, and will advance understanding of viral assembly and disassembly, viral-host interactions and specificity, and viral transmission, as well as the relationship between structure and the characteristically rapid evolution of RNA viruses. ***