Structure and Assembly of Helical Plant Viruses

螺旋植物病毒的结构和组装

• 批准号: 9506204
• 负责人: Gerald Stubbs
• 金额: $ 30万
• 依托单位: Vanderbilt University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-11-01 至 1999-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9506204&HistoricalAwards=false
• 关键词: Structure; Assembly; Helical; Plant; Viruses

9506204 Stubbs The structures of a number of helical plant viruses will be determined in molecular detail, using the fiber diffraction methods already used in this laboratory for several viruses of the tobamovirus (tobacco mosaic virus, TMV) group. The viruses to be studied include three tobamoviruses, odontoglossum ringspot virus (ORSV), ribgrass mosaic virus (RMV) and sunn-hemp mosaic virus (SHMV), and one furovirus, soil-borne wheat mosaic virus (SBWMV). Structures of the three tobamoviruses will be determined by molecular replacement, using the known structure of TMV. The structure of SBWMV will be determined using the methods of multi- dimensional isomorphous replacement developed in this laboratory. All structures will be refined using molecular dynamics methods, as adapted in this laboratory for use with fiber diffraction data. These refinement methods will be developed further. The tobamovirus group is the only rod-shaped virus group for which three-dimensional structures have been determined at the molecular level. The structures to be determined will be important in understanding the molecular characteristics of the group, including the relationship between subgroups, and will advance understanding of viral assembly and disassembly, viral-host interactions and specificity, and vector transmission, as well as the relationship between structure and the rapid evolution of RNA viruses. %%% The structures of a number of rod-shaped plant viruses will be determined in molecular detail, using the fiber diffraction methods already used in this laboratory for several viruses of the tobamovirus. The viruses to be studied include three tobamoviruses, odontoglossum ringspot virus (ORSV), ribgrass mosaic virus (RMV) and sunn-hemp mosaic virus (SHMV), and the furovirus soil-borne wheat mosaic virus (SBWMV). The tobamovirus group is the only rod- shaped virus group for which three-dimensional structures have been determined at the molecular level. The structu res to be determined will be important in understanding the molecular characteristics of the group, including the relationship between subgroups, and will advance understanding of viral assembly and disassembly, viral-host interactions and specificity, and viral transmission, as well as the relationship between structure and the characteristically rapid evolution of RNA viruses. ***

许多螺旋状植物病毒的结构将用本实验室已用于烟草花叶病毒（tobacco mosaic virus，TMV）组的几种病毒的纤维衍射方法在分子上详细测定。 待研究的病毒包括齿舌草环斑病毒（ORSV）、牛筋草花叶病毒（RMV）和大麻花叶病毒（SHMV）三种烟草花叶病毒和土传小麦花叶病毒（SBWMV）一种糠病毒。 将使用TMV的已知结构通过分子置换来确定三种烟草花叶病毒的结构。 SBWMV的结构将使用本实验室开发的多维同晶置换方法来确定。 所有的结构都将使用分子动力学方法进行优化，如本实验室所采用的纤维衍射数据。 这些改进方法将得到进一步发展。 杆状病毒组是唯一的杆状病毒组，其三维结构已在分子水平上确定。 待确定的结构对于理解该组的分子特征（包括亚组之间的关系）将是重要的，并将促进对病毒组装和拆卸、病毒-宿主相互作用和特异性、载体传播以及结构与RNA病毒快速进化之间的关系的理解。 一些杆状植物病毒的结构将用本实验室已用于几种杆状病毒的纤维衍射方法进行分子细节的测定。待研究的病毒包括三种烟草花叶病毒，齿舌草环斑病毒（ORSV）、牛筋草花叶病毒（RMV）和阳光大麻花叶病毒（SHMV），以及糠病毒土传小麦花叶病毒（SBWMV）。 杆状病毒组是唯一在分子水平上确定了三维结构的杆状病毒组。 待确定的结构对于理解该组的分子特征（包括亚组之间的关系）将是重要的，并且将促进对病毒组装和拆卸、病毒-宿主相互作用和特异性、病毒传播以及RNA病毒的结构与特征性快速进化之间的关系的理解。 ***