Structure, Assembly, and Host Interactions of Helical Plant Viruses

M9809879 Stubbs许多丝状植物病毒的结构将使用纤维衍射方法在分子细节上确定。 待研究的病毒包括两种麻花叶病毒，齿舌兰环斑病毒（ORSV）和日光大麻花叶病毒（SHMV），以及糠病毒、马铃薯X病毒和马铃薯Y病毒组的成员。 烟草花叶病毒的结构将使用烟草花叶病毒（TMV）的已知结构通过分子置换来确定。 其他结构将使用P.I.的实验室。 所有的结构都将使用分子动力学方法进行优化，如P.I.的实验室用于纤维衍射数据。 这些结构的确定对于理解病毒的分子特征将是重要的，并将促进对病毒组装和拆卸、病毒-宿主相互作用和特异性、载体传播以及RNA病毒的结构与快速进化之间的关系的理解。 待研究的病毒包括两种麻花叶病毒，齿舌兰环斑病毒（ORSV）和日光大麻花叶病毒（SHMV），以及糠病毒、马铃薯X病毒和马铃薯Y病毒组的成员。 烟草花叶病毒和马铃薯X病毒在生物技术中的重要性日益增加。 马铃薯X病毒属，特别是马铃薯Y病毒属在农业上具有重要意义。

M9809879StubbsThe structures of a number of filamentous plant viruses will be determined in molecular detail, using fiber diffraction methods. The viruses to be studied include two tobamoviruses, odontoglossum ringspot virus (ORSV) and sunn-hemp mosaic virus (SHMV), and members of the furovirus, potexvirus, and potyvirus groups. Structures of the tobamoviruses will be determined by molecular replacement, using the known structure of tobacco mosaic virus (TMV). The other structures will be determined de novo, using methods developed in P.I.'s laboratory. All structures will be refined using molecular dynamics methods, as adapted in P.I.'s laboratory for use with fiber diffraction data. The structures to be determined will be important in understanding the molecular characteristics of viruses, and will advance understanding of viral assembly and disassembly, viral-host interactions and specificity, and vector transmission, as well as the relationship between structure and the rapid evolution of RNA viruses.The structures of a number of filamentous plant viruses will be determined using fiber diffraction methods. The viruses to be studied include two tobamoviruses, odontoglossum ringspot virus (ORSV) and sunn-hemp mosaic virus (SHMV), and members of the furovirus, potexvirus, and potyvirus groups. Tobamoviruses and potexviruses are of increasing importance in biotechnology. Potexviruses and especially potyviruses are of major agricultural importance.