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Structure, Assembly, and Host Interactions of Helical Plant Viruses

螺旋植物病毒的结构、组装和宿主相互作用

基本信息

批准号：
9809879
负责人：
Gerald Stubbs
金额：
$ 33万
依托单位：
Vanderbilt University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1998
资助国家：
美国
起止时间：
1998-11-01 至 2002-10-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9809879&HistoricalAwards=false
关键词：
Structure Assembly Host Interactions Helical

项目摘要

M9809879StubbsThe structures of a number of filamentous plant viruses will be determined in molecular detail, using fiber diffraction methods. The viruses to be studied include two tobamoviruses, odontoglossum ringspot virus (ORSV) and sunn-hemp mosaic virus (SHMV), and members of the furovirus, potexvirus, and potyvirus groups. Structures of the tobamoviruses will be determined by molecular replacement, using the known structure of tobacco mosaic virus (TMV). The other structures will be determined de novo, using methods developed in P.I.'s laboratory. All structures will be refined using molecular dynamics methods, as adapted in P.I.'s laboratory for use with fiber diffraction data. The structures to be determined will be important in understanding the molecular characteristics of viruses, and will advance understanding of viral assembly and disassembly, viral-host interactions and specificity, and vector transmission, as well as the relationship between structure and the rapid evolution of RNA viruses.The structures of a number of filamentous plant viruses will be determined using fiber diffraction methods. The viruses to be studied include two tobamoviruses, odontoglossum ringspot virus (ORSV) and sunn-hemp mosaic virus (SHMV), and members of the furovirus, potexvirus, and potyvirus groups. Tobamoviruses and potexviruses are of increasing importance in biotechnology. Potexviruses and especially potyviruses are of major agricultural importance.

M9809879Stubbs将使用纤维衍射方法确定许多丝状植物病毒的分子细节。待研究的病毒包括两种烟草花叶病毒、牙舌兰环斑病毒（ORSV）和太阳麻花叶病毒（SHMV），以及糠秕病毒、马痘病毒和马铃薯病毒组的成员。烟草花叶病毒的结构将利用已知的烟草花叶病毒（TMV）结构通过分子置换来确定。其他结构将使用 P.I. 实验室开发的方法从头确定。所有结构都将使用分子动力学方法进行精炼，这些方法在 P.I. 实验室中进行了调整，用于纤维衍射数据。待确定的结构对于了解病毒的分子特征非常重要，并将促进对病毒组装和分解、病毒与宿主相互作用和特异性、载体传播以及RNA病毒的结构和快速进化之间的关系的理解。许多丝状植物病毒的结构将利用纤维衍射方法确定。待研究的病毒包括两种烟草花叶病毒、牙舌兰环斑病毒（ORSV）和太阳麻花叶病毒（SHMV），以及糠秕病毒、马痘病毒和马铃薯病毒组的成员。烟草花叶病毒和马铃薯病毒在生物技术中越来越重要。马铃薯病毒属，尤其是马铃薯病毒属具有重要的农业重要性。