Development of Methods in Fiber Diffraction Analysis of Macromolecules

许多大分子产生衍射图案时， 暴露在X光下 以前，这些模式用于 确定分子的分子结构， 重复部分，否则分析仅限于非常低的 分辨率 在过去的15年里，首席研究员 开发了X射线衍射分析方法（称为纤维 衍射分析），其已经确定了分子 烟草花叶病毒（TMVB）的结构，分辨率为 2.9埃。 目前的提议是制定方法， 将TMV研究中使用的技术扩展到其他 生物系统。 将使用其他方法制定方法 病毒、细胞骨架丝和核蛋白复合物。

Many macro molecules give rise to diffraction patterns when exposed to X-rays. Previously, these patterns were of use in determining molecular structure of molecules which contain small, repeating portions or else the analysis was limited to very low resolution. Over the lst l5 years, the principal investigator has developed methods of X-ray diffraction analysis (called fiber diffraction analysis) which have determined the molecular structure of the Tobacco Mosaic Virus (TMVB) at a resolution of 2.9 angstroms. The present proposal is to develop methods to extend the techniques utilized in the TMV study to other biological systems. Methodology will be developed using other viruses, cytoskeletal filaments and nucleoprotein complexes.