Purchase of Equipment for Protein Crystallography, Fiber Diffraction and Cryo-electron

建议购买一台Optronics C-4100SP微密度计和一台Silicon Graphics Iris 4D/280SX计算机，用于蛋白质和核酸的结构研究。斯塔布斯，安德斯，弗莱克，罗宾逊，纽卡姆和其他人。Drs。Flicker和Robinson计划在蛋白质组合的结构研究中使用低温电子显微镜：Flicker博士正在研究肌动球蛋白复合物；罗宾逊博士研究神经毒素。斯塔布斯博士对植物病毒结构的研究也将受益于冷冻电子显微镜设备。Drs。安德森、纽卡姆和斯塔布斯正在使用蛋白质晶体学方法来研究各种分子。随着一些新的晶体的出现，现有的设备已经达到饱和。此外，一些晶体形式的晶胞太大，无法使用现有设施收集数据。斯塔布斯博士的纤维衍射研究还需要能够从非常大的单晶胞中解析衍射数据的设备。主要用户大量使用现有的电脑设施。结构的细化，特别是安德森博士和斯塔布斯博士以及纽卡姆博士和弗利克博士的新项目，将需要大幅提高计算能力。

It is proposed that an Optronics C-4100SP microdensitometer, and a Silicon Graphics Iris 4D/280SX computer be purchased, for use in structural studies of proteins and nucleic acids by Drs. Stubbs, Anders, Flicker, Robinson, Newcomer and others. Drs. Flicker and Robinson plan to utilize cryo-electron microscopy in their structural studies of protein assemblies: Dr. Flicker is studying actomyosin complexes; Dr. Robinson studies neurotoxins. Dr. Stubbs' studies of plant virus structure will also benefit from cryo-electron microscopy facilities. Drs. Anderson, Newcomer, and Stubbs are using protein crystallographic methods to study a variety of molecules. With a number of new crystals new available, the present facilities have reached saturation. Furthermore, several of the crystal forms have unit cells too large to permit data collection using existing facilities. Dr. Stubbs' fiber diffraction studies also require equipment capable of resolving diffraction data from very large unit cells. The major users make heavy use of the existing computer facilities. Structural refinement, especially by Dr. Anderson and Stubbs and new projects of Dr. Newcomer and Flicker, will require substantially increased computing capacity.