Purchase of Equipment for Protein Crystallography, Fiber Diffraction and Cryo-electron

建议采用Optronics C-4100 SP显微密度计， 购买Silicon Graphics Iris 4D/280 SX计算机，用于 斯塔布斯博士对蛋白质和核酸的结构研究， 安德斯、弗里克、罗宾逊、新来的人和其他人。 弗里克博士和罗宾逊博士计划利用低温电子显微镜 在他们的蛋白质组装结构研究中： 研究肌动球蛋白复合物;罗宾逊博士研究神经毒素。 斯塔布斯博士对植物病毒结构的研究也将受益于 冷冻电子显微镜设备。 安德森、纽卡默和斯塔布斯博士正在使用蛋白质 晶体学方法来研究各种分子。 与 一些新的晶体新的可用，目前的设施有 达到饱和。 此外，几种晶体形式具有 单位单元太大，无法使用现有的 设施 斯塔布斯博士的纤维衍射研究还需要 一种能分辨大尺寸衍射数据的设备， 单位细胞 主要用户大量使用现有的计算机设施。 结构的改进，特别是安德森博士和斯塔布斯， 新来的博士和闪烁的新项目，将需要 大大提高了计算能力。

It is proposed that an Optronics C-4100SP microdensitometer, and a Silicon Graphics Iris 4D/280SX computer be purchased, for use in structural studies of proteins and nucleic acids by Drs. Stubbs, Anders, Flicker, Robinson, Newcomer and others. Drs. Flicker and Robinson plan to utilize cryo-electron microscopy in their structural studies of protein assemblies: Dr. Flicker is studying actomyosin complexes; Dr. Robinson studies neurotoxins. Dr. Stubbs' studies of plant virus structure will also benefit from cryo-electron microscopy facilities. Drs. Anderson, Newcomer, and Stubbs are using protein crystallographic methods to study a variety of molecules. With a number of new crystals new available, the present facilities have reached saturation. Furthermore, several of the crystal forms have unit cells too large to permit data collection using existing facilities. Dr. Stubbs' fiber diffraction studies also require equipment capable of resolving diffraction data from very large unit cells. The major users make heavy use of the existing computer facilities. Structural refinement, especially by Dr. Anderson and Stubbs and new projects of Dr. Newcomer and Flicker, will require substantially increased computing capacity.