Regulation of Membrane Lipid Hydrolysis in Lipid-based Signaling of Plants

9511623 Wang通过十八烷酸途径的基于脂质的信号传导，其导致调节剂如茉莉酸的形成，在植物生理过程中起重要作用。 茉莉酸和相关的含氧脂质已被证明是激活防御基因表达和调节生长和发育的内源性信号。 α-亚麻酸已被证明是合成这些调节剂的前体。 然而，很少有人知道α-亚麻酸在十八烷信号通路中的脂解释放。 哪种脂类水解酶在信号传导过程中被激活？ 哪些甘油脂会水解？ 如何调节水解？ 这些问题很重要，因为脂肪酸前体是信号转导过程中的早期步骤，并可能控制信号通路。 因此，本研究的目的是调查十八烷酸信号通路中脂解酶脱酰作用的参与、性质和调节。 刺激诱导的脂质周转和释放的α-亚麻酸将在蓖麻叶中响应创伤进行研究。 选择创伤是因为它是诱导茉莉酸信号传导系统的充分表征的刺激。 这项工作的完成将填补我们对膜脂水解酶在植物生长和环境响应中的细胞作用的认识中的一个关键空白。

9511623 Wang Lipid-based signaling via the octadecanoid pathway, which leads to the formation of regulators such as jasmonic acid, plays an important role in plant physiological processes. Jasmonate and related oxygenated lipids have been shown to act as endogenous signals activating defense gene expression and regulating growth and development. Alph-linolenic acid has been shown to be the precursor for the synthesis of these regulators. However, very little is known about lipolytic release of alpha-linolenic acid in the octadecanoid signaling pathway. Which lipid hydrolyzing enzyme is activated in the signaling process? Which glycerolipids are hydrolyzed? How is the hydrolysis regulated? These questions are important because making available the fatty acid precursor is an early step in the sequence of events in signal transduction and may control the signaling pathway. The goal of this research is, therefore, to investigate the involvement, nature, and regulation of deacylation by lipolytic enzymes in the octadecanoid signaling pathway. Stimulus-induced lipid turnover and release of alpha-linolenic acid will be investigated in castor bean leaves in response to wounding. Wounding is chosen because it is a well characterized stimulus that induces the jasmonate signaling system. Completion of the proposed work will fill a critical gap in our knowledge of the cellular roles of membrane lipid-hydrolyzing enzymes in plant growth and environmental responsiveness.