Metabolomic Profiling of Membrane Lipids and their Compositional Dynamics in Plant Stress Responses

膜脂质的代谢组学分析及其在植物胁迫反应中的组成动态

• 批准号: 0110979
• 负责人: Xuemin Wang
• 金额: $ 45万
• 依托单位: Kansas State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2005-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0110979&HistoricalAwards=false
• 关键词: Metabolomic; Profiling; Membrane; Lipids; their

Membrane lipids comprise diverse molecular species, and their composition differs from membrane to membrane. In addition, membrane lipid composition changes in response to internal and external cues. Furthermore, within a membrane, there may be microdomains with distinct lipid constituents and particular functions. However, it is not understood how these distinct compositions and their dynamics are generated and what their functions are in the cell. The objectives of this project are to use a metabolomic approach to determine cellular membrane lipid composition and to understand the regulation and role of membrane lipid compositional dynamics in plant responses to stresses. A highly sensitive approach based on electrospray ionization tandem mass spectrometry (ESI-MS/MS) will be established. ESI-MS/MS will be employed to profile membrane lipid molecular species and to determine the compositional dynamics in Arabidopsis plants undergoing temperature and drought stresses. To understand how lipid changes are regulated, this project will investigate enzymes involved in generating the membrane lipid compositional dynamics. Arabidopsis lines, abrogated of various isoforms of phospholipase D, the major lipolytic enzyme family, will be instrumental in the analysis. In addition, lipid molecular species of the defense mutant ssi2 that is defective in stearoyl-ACP desaturase and its suppressor lines will be profiled to determine the relationship between lipid composition and alterations in defense responses. The capability to combine full lipid profiling with cellular analysis of the machinery that generates compositional changes should yield new information on how cellular machinery and metabolites interact in a dynamic manner in the cellular response to changing environments. Membrane lipids are vital biological constituents, providing structural backbones for biological membranes and crucial resources for producing second messengers in regulating cellular and organismal functions. Membrane lipids comprise diverse molecular species, and the composition differs from membrane to membrane. The lipid composition changes in response to internal and external cues. However, it is not understood how these distinct compositions and their dynamics are generated and what their functions are in the cell. This project will establish a highly effective approach based on electrospray ionization tandem mass spectrometry and use it to determine cellular membrane lipid composition and the role of membrane lipid compositional changes in plant responses to stresses. Extensive profiling of membrane lipids and their metabolites will yield unprecedented information on how cellular machinery and metabolites interact in a dynamic manner in the cellular response to changing environments.

膜脂质包括不同的分子种类，并且它们的组成在膜与膜之间不同。 此外，膜脂组成的变化响应内部和外部线索。此外，在膜内，可能存在具有不同脂质成分和特定功能的微区。然而，目前还不清楚这些不同的成分及其动力学是如何产生的，以及它们在细胞中的功能是什么。 本项目的目标是使用代谢组学的方法来确定细胞膜脂组成，并了解膜脂组成动态的调节和作用，在植物对胁迫的反应。将建立一种基于电喷雾串联质谱（ESI-MS/MS）的高灵敏度方法。ESI-MS/MS将被用来分析膜脂分子种类，并确定拟南芥植物经历温度和干旱胁迫的组成动态。为了了解脂质变化是如何调节的，本项目将研究参与产生膜脂质成分动态的酶。拟南芥系，废除各种异构体的磷脂酶D，主要的脂肪分解酶家族，将有助于分析。此外，脂质分子种类的防御突变体的ssi 2是有缺陷的硬脂酰-ACP去饱和酶及其抑制系将被分析，以确定脂质组成和防御反应的变化之间的关系。将联合收割机全脂质谱分析与产生组成变化的机器的细胞分析相结合的能力应产生关于细胞机器和代谢物如何在细胞对变化的环境的响应中以动态方式相互作用的新信息。 膜脂是重要的生物组分，为生物膜提供结构骨架，并为产生调节细胞和生物体功能的第二信使提供重要资源。膜脂质包括不同的分子种类，并且膜与膜的组成不同。脂质组成的变化响应内部和外部线索。然而，目前还不清楚这些不同的成分及其动力学是如何产生的，以及它们在细胞中的功能是什么。该项目将建立一种基于电喷雾串联质谱的高效方法，并使用它来确定细胞膜脂组成以及膜脂组成变化在植物应对胁迫中的作用。 膜脂质及其代谢物的广泛分析将产生前所未有的信息，细胞机械和代谢物如何在细胞对不断变化的环境的反应中以动态的方式相互作用。