U.S.-New Zealand Cooperative Research: Pulse Radiolysis Studies of Xanthine Oxidase and Trimethylamine Dehydrogenase

该奖项将支持俄亥俄州立大学医学生物化学系的Charles Russell Hille博士与奥克兰大学化学系的Robert Anderson博士进行为期三年的合作研究。合作研究将集中于两种复合氧化还原酶内电子转移的表征。来自牛奶的黄嘌呤氧化酶催化黄嘌呤氧化羟基化形成尿酸。该酶的四个氧化活性中心的研究将采用脉冲辐射分解法进行。其他工作将集中在三甲胺脱氢酶内的电子转移。脉冲辐射分解数据将用于比较和对比这些蛋白质在观察到的电子转移过程中涉及的原生事件的行为。奥克兰的脉冲辐射分解设施是专门为生物应用而设计的。该项目将希尔博士的生化专业知识与安德森博士实验室的设备结合在一起。

9513747 Hille This award will support the travel over three years to enable Dr. Charles Russell Hille from the Department of Medical Biochemistry at the Ohio State University to conduct collaborative research with Dr. Robert Anderson of the Department of Chemistry at the University of Auckland. The cooperative research will focus on the characterization of electron transfer within two complex oxidoreductases. Xanthine oxidase from cow's milk catalyses the oxidative hydroxylation of Xanthine to form uric acid. Studies of the four redox-active centers of this enzyme will be pursued using pulse radiolysis. Other work will focus on electron transfer within trimethylamine dehydrogenase. The pulse radiolysis data will be used to compare and contrast the behavior of these proteins with regard to the involvement of prototropic events in the observed electron transfer processes. The pulse radiolysis facilities in Auckland have been specifically designed with biological applications in mind. This project draws together the biochemical expertise of Dr. Hille with the facilities available in Dr. Anderson's laboratory.