U.S.-New Zealand Cooperative Research: Pulse Radiolysis Studies of Xanthine Oxidase and Trimethylamine Dehydrogenase

美国-新西兰合作研究：黄嘌呤氧化酶和三甲胺脱氢酶的脉冲放射分解研究

• 批准号: 9513747
• 负责人: Charles Hille
• 金额: $ 1.8万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9513747&HistoricalAwards=false
• 关键词: U.S.; New; Zealand; Cooperative; Research

9513747 Hille This award will support the travel over three years to enable Dr. Charles Russell Hille from the Department of Medical Biochemistry at the Ohio State University to conduct collaborative research with Dr. Robert Anderson of the Department of Chemistry at the University of Auckland. The cooperative research will focus on the characterization of electron transfer within two complex oxidoreductases. Xanthine oxidase from cow's milk catalyses the oxidative hydroxylation of Xanthine to form uric acid. Studies of the four redox-active centers of this enzyme will be pursued using pulse radiolysis. Other work will focus on electron transfer within trimethylamine dehydrogenase. The pulse radiolysis data will be used to compare and contrast the behavior of these proteins with regard to the involvement of prototropic events in the observed electron transfer processes. The pulse radiolysis facilities in Auckland have been specifically designed with biological applications in mind. This project draws together the biochemical expertise of Dr. Hille with the facilities available in Dr. Anderson's laboratory.

小行星9513747 该奖项将支持为期三年的旅行，使俄亥俄州州立大学医学生物化学系的Charles Russell Hille博士能够与奥克兰大学化学系的Robert安德森博士进行合作研究。 合作研究将集中在两个复杂的氧化还原酶内的电子转移的表征。 来自牛奶的黄嘌呤氧化酶催化黄嘌呤的氧化羟基化形成尿酸。 研究的四个氧化还原活性中心的这种酶将采用脉冲辐解。 其他工作将集中在三甲胺脱氢酶内的电子转移。 脉冲辐解数据将被用来比较和对比这些蛋白质的行为，在所观察到的电子转移过程中的质子转移事件的参与。 奥克兰的脉冲辐解设施是专门为生物应用而设计的。 该项目将Hille博士的生物化学专业知识与安德森博士实验室的设施结合在一起。