Acquisition of an Electron Paramagnetic Resonance Spectrometer

电子顺磁共振光谱仪将需要在广泛的生物学问题的研究。该仪器的应用包括：（1）黄嘌呤氧化酶和亚硫酸盐氧化酶钼中心的结构和机理研究;（2）质体蓝素化学修饰对其铜中心结构和功能的影响研究;（3）普通脱硫弧菌黄素氧还蛋白基因工程形式的氧化还原电位测定;（4）底物与黄嘌呤氧化酶结合的自旋标记研究，目的是确定黄嘌呤与酶的氧化形式结合的Kd和活性位点中存在的底物结合位点的数目，（5）利用激光诱导激发一氧化碳细胞色素c氧化酶和亚硝基血红蛋白来检测这些中心的EPR信号的变化。总之，这些项目构成了电子顺磁共振光谱学的一个主要应用，这将大大提高俄亥俄州州立大学生物化学家扩展组的基础研究能力。

An electron paramagnetic resonance spectrometer will be required for studies in a wide range of biological problems. Applications of the instrument include: (1) structural and mechanistic studies of the molybdenum centrs of xanthine oxidase ans sulfite oxidase, (2) studies of the effects of chemical modificatio of plastocyanin on the structure and function of its copper center, (3) determination of the oxidation-reduction potentials of genetically engineered forms of the flavodoxin from Desulfovibrio vulgaris, (4) a spin-label study of substrate binding to xanthine oxidase aimed at establishing the Kd for binding of xanthine to the oxidized form of the enzyme and the number of substrate binding sites present in the active site, (5) use of laser-induced excitation of carbonmonoxy cytochrome c oxidase and nitrosylhemoglobin to examine changes in the EPR signals of these centers. Together, these projects constitute a major application of elctron paramagnetic resonance spectroscopy that would greatly enhance the basic research capability of an extended group of biochemists at Ohio State University.