Identification of Host Factors Required for Replication of Geminivirus DNA

双生病毒 DNA 复制所需宿主因子的鉴定

• 批准号: 9601893
• 负责人: Dominique Robertson
• 金额: $ 20万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9601893&HistoricalAwards=false
• 关键词: Identification; Host; Factors; Required; Replication

9601893 Robertson Tomato golden mosaic virus (TGMV) replicates in nuclei of cells which have excited the cell cycle. Proliferating cell nuclear antigen(PCNA), an accessory factor for DNA polymerase gamma, was co-localized with TGMV in infected cells but was absent in comparable, differentiated cells of healthy plants. PCNA is the first example of a host protein associated with geminivirus infections. The specific goals of this work are: 1) To determine if TGMV infection causes the accumulation of cell cycle regulatory proteins 2) To determine if the viral replication protein, AL1, causes an increase in the expression of other host proteins and 3)To determine if TGMV DNA is preferentially replicated or if chromosomal DNA is also replicated. %%% The results of this investigation should lead to important insights into the mechanisms and possible linkage of viral pathogenesis and cell cycle regulation in the host plant. New approaches for the control of viral plant diseases may be developed from the enhanced understanding of such interactions. ***

9601893罗伯逊番茄黄金花叶病毒(TGMV)在细胞核内复制，刺激细胞周期。增殖细胞核抗原是DNA聚合酶γ的一个辅助因子，在感染细胞中与TGMV共定位，但在健康植物的可比分化细胞中缺失。增殖细胞核抗原是第一个与双生病毒感染相关的宿主蛋白的例子。这项工作的具体目标是：1)确定TGMV感染是否导致细胞周期调节蛋白的积累；2)确定病毒复制蛋白AL1是否导致其他宿主蛋白表达增加；3)确定TGMV DNA是优先复制还是染色体DNA也复制。这项研究的结果将对病毒致病机制和寄主植物细胞周期调控的可能联系有重要的指导意义。通过加强对这种相互作用的了解，可以开发出控制病毒植物疾病的新方法。***