Molecular Analysis of Tobacco Phloem-Specific Protein

烟草韧皮部特异性蛋白的分子分析

• 批准号: 9306608
• 负责人: Dominique Robertson
• 金额: $ 1.8万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9306608&HistoricalAwards=false
• 关键词: Molecular; Analysis; Tobacco; Phloem; Specific

9306608 Robertson Phloem-specific protein (p-protein) is a major protein in sieve tube elements, yet the function of this protein is not known. Functions commonly ascribed to p-protein are that it plugs sieve pore plates following wounding and/or that it maintains sterility in phloem by binding microorganisms. However, the synthesis and turnover of p-protein are very high, characteristics not normally associated with proteins that are primarily structural in nature. In the electroosmotic flow theory of phloem translocation, p- protein has been hypothesized to have an active role in the transport of photosynthetic assimilates. Antisense technology to phenocopy null mutations for p-protein isoforms can help to clarify the role of p-protein in translocation. As the first step towards this goal, the PI proposes to isolate p-protein genes from tobacco, Arabidopsis and pumpkin. %%% A long term goal is to monitor changes in translocation flow and in the plant's response to wounding in plants transformed with antisense p-protein mRNA. These experiments will increase our understanding of the mechanism of phloem translocation and will help elucidate the function and importance of p-protein in normal plant growth. The knowledge gained from such studies will increase our basic understanding of phloem tissues and may have important consequences for agriculture. ***

9306608罗伯逊韧皮部特异蛋白(p-蛋白)是筛管分子中的一种主要蛋白质，但其功能尚不清楚。通常归因于p-蛋白的功能是，它在受伤后堵塞筛孔孔板和/或通过结合微生物来维持韧皮部的不育性。然而，p-蛋白质的合成和周转非常高，这些特征通常与主要是结构性质的蛋白质不相关。在韧皮部运输的电渗流理论中，p蛋白被认为在光合作用同化物的运输中起着积极的作用。P-蛋白亚型表型零突变的反义技术有助于阐明p-蛋白在易位中的作用。作为实现这一目标的第一步，PI提议从烟草、拟南芥和南瓜中分离p-蛋白基因。一个长期的目标是监测转反义p-蛋白mRNA的植物中易位流动的变化和植物对伤害的反应。这些实验将加深我们对韧皮部运输机制的理解，并将有助于阐明p-蛋白在植物正常生长中的功能和重要性。从这些研究中获得的知识将增加我们对韧皮部组织的基本了解，并可能对农业产生重要影响。***