EAGER: Molecular Identification of a Geminivirus Resistance Gene in Arabidopsis

EAGER：拟南芥双生病毒抗性基因的分子鉴定

• 批准号: 1049794
• 负责人: Dominique Robertson
• 金额: $ 12.08万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1049794&HistoricalAwards=false
• 关键词: EAGER; Molecular; Identification; Geminivirus; Resistance

Geminiviruses are plant DNA viruses that cause severe economic damage to tomato, cotton, corn, and other crop plants, primarily in tropical regions of the world. They are considered to be an emerging threat because of recent outbreaks that caused wide-spread famine in Uganda (cassava) and other parts of Africa (corn) and economic hardship in Pakistan and India (cotton). They are a threat to the US because of the recent introduction of Tomato yellow leaf curl virus from Israel and the possible introduction of Old World geminiviruses infecting cotton or corn. Although resistance genes for different geminiviruses have been identified in bean, tomato, corn, and other crops, none of them has been sequenced. Sequencing of a potyvirus resistance gene in Arabidopsis was a breakthrough for plant breeding because it allowed the identification of naturally occurring resistance genes in several different crop plants and also shed light on exactly which host factors were important for RNA virus infection. An Arabidopsis gene has been identified that confers resistance to two very different types of DNA virus. Such broad-based resistance would be an important complement to genetic engineering methods, such as RNAi, that are powerful but virus-specific. The goal of this project is to identify and sequence the Arabidopsis geminivirus resistance gene by determining which DNA fragments correlate with resistance in a mapping (F2) population. A cloned gene corresponding to this DNA fragment will be transformed into a susceptible plant and challenged with both geminiviruses. If the gene behaves as expected (resistance is conferred), information about the new function of the gene will be submitted to the Arabidopsis Information Resource (TAIR) and then published. This project will support a Hispanic postdoctoral student for one year.

双生病毒是一种植物DNA病毒，主要在世界热带地区对番茄、棉花、玉米和其他农作物造成严重的经济损害。它们被认为是一个新的威胁，因为最近的疫情在乌干达(木薯)和非洲其他地区(玉米)造成了大范围的饥荒，在巴基斯坦和印度(棉花)造成了经济困难。它们对美国构成威胁，因为最近从以色列引进了番茄黄化卷叶病毒，并可能引入感染棉花或玉米的旧大陆双生病毒。虽然已经在豆类、番茄、玉米和其他作物中鉴定出了不同双生病毒的抗性基因，但还没有对它们进行测序。拟南芥中一个马铃薯Y病毒抗性基因的测序是植物育种的一项突破，因为它可以鉴定几种不同作物中自然产生的抗性基因，并准确地揭示哪些寄主因素对RNA病毒的侵染起重要作用。拟南芥的一种基因已经被鉴定出来，它可以抵抗两种截然不同的DNA病毒。这种广泛的抗药性将是对基因工程方法的重要补充，例如RNAi，这些方法功能强大，但具有病毒特异性。该项目的目标是通过确定在作图(F2)群体中哪些DNA片段与抗性相关来鉴定拟南芥双生病毒抗性基因并对其进行测序。与该DNA片段相对应的克隆基因将被转化到敏感植物中，并与这两种双生病毒共同攻击。如果该基因的行为与预期一致(抗药性被赋予)，有关该基因新功能的信息将被提交给拟南芥信息资源(TAIR)，然后发表。该项目将支持一名西班牙裔博士后一年。