Automated Molecular Anthropological Analysis

自动分子人类学分析

• 批准号: 9602171
• 负责人: Todd Disotell
• 金额: $ 4.48万
• 依托单位: New York University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-15 至 1997-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9602171&HistoricalAwards=false
• 关键词: Automated; Molecular; Anthropological; Analysis

With National Science Foundation support Dr. Todd Disotell and his colleagues will purchase an ABI PRISM 310 Genetic Analyzer. This instrument is an automated system used for the sizing, sequencing and quantification of nucleic acids. The device integrates multicolor fluorescent labeling of nucleic acids with capillary electrophoresis to size and quantitate DNA molecules. Two of the advantages of using this technology to measure DNA include the use of polymers in capillary tubes rather than gels and the use of fluorescent dyes rather than radioactive isotopes for visualization. The instrument has a daily throughput of up to 4200 DNA bases when used for sequencing and is fully automated, so that once samples are prepared they are automatically loaded, run and analyzed without additional user input. This is a great advantage over manual techniques that need to be continuously monitored. The goal of the research is to understand the processes involved in the evolution of higher primate species and the instrument will be used to sequence multiple loci from large numbers of individuals from select primate species. In particular the researchers will focus on closely related Cercopithecines (guenons, macaques, mangabeys and mandrills) and on two species of baboons which interbreed in a hybrid zone. The project is especially interesting because it focuses on species that are just on the cusp of evolutionary separation and therefore the work will shed light on both the behavioral and biological mechanisms which serve to establish interspecific barriers. Intellectually the project falls at the borderline between population genetics and taxonomic analysis and serves to integrate concepts from both areas. Acquisition of this instrument will speed research in Dr. Disotell's lab and generate data of interest to many biologists and social scientists. It will also assist in the training of students in the NYU and other New York City university anthropology and biology departments.

随着国家科学基金会的支持，托德·索特尔（Todd DiSotell）博士及其同事将购买ABI Prism 310遗传分析仪。该仪器是一种自动化系统，用于核酸的大小，测序和定量。该设备将核酸与毛细管电泳的多色荧光标记集成到大小并定量DNA分子。使用该技术测量DNA的两个优点包括在毛细管中使用聚合物而不是凝胶，以及使用荧光染料而不是放射性同位素进行可视化。该仪器的每日吞吐量在用于测序时最多为4200个DNA碱基，并且是完全自动化的，因此一旦准备好样品，它们就会自动加载，运行和分析，而无需其他用户输入。这是需要不断监控的手动技术的巨大优势。 该研究的目的是了解较高灵长类动物物种进化的过程，该仪器将用于对来自精选灵长类动物的大量个体的多个基因座进行序列。特别是，研究人员将专注于密切相关的Cercopithecines（圭山，猕猴，芒果和Mandrills），以及两种在混合区域中杂交的狒狒。该项目特别有趣，因为它专注于仅在进化分离的风口浪尖上，因此该工作将阐明有助于建立种间屏障的行为和生物学机制。从智力上讲，该项目位于人口遗传学和分类学分析之间的边界，并有助于整合两个领域的概念。 获取该工具将加快Disotell博士实验室的研究，并为许多生物学家和社会科学家生成感兴趣的数据。它还将协助培训纽约大学和其他纽约大学人类学和生物学系的学生。