Interdepartmental (Biology, Anthropology, Chemistry) Genetic Analysis Facility

在美国国家科学基金会的支持下，Todd Disotell博士和他在纽约大学的合作者将购买ABI PRISM 377系统，该系统由电泳和检测单元，Power Macintosh计算机和用于DNA测序分析或PCR片段大小和定量的软件组成。该系统将通过使用多色荧光技术实现高通量，通过在同一车道上共同加载多个PCR或测序反应的产物，允许多次电泳。为了提高利用科学家小组的效率，将获得热循环仪（Perkin-Elmer Geneamp PCR System 9700）以及微离心机（Eppendorf 5400系列）和微浓缩仪（Savant DNA SpeedVac），并与ABI遗传分析仪一起安置。一台Power Macintosh计算机将允许在ABI基因分析仪上收集的数据进行离线分析，允许控制ABI机器的计算机专门用于数据收集。这种仪器将大大提高六个研究项目的效率，这些项目由许多研究人员进行。这些研究包括1。横纹肌线虫形态发生和形态进化的遗传发育机制2. 秀丽隐杆线虫性腺器官发生遗传学；3.灵长类动物分子进化与群体遗传学；4. 鱼类洞穴适应的进化遗传学研究裂变酵母中与Ras - g蛋白和微管相互作用的未知蛋白质；和6。HMG蛋白识别DNA结构。这台机器也将用于研究生和本科生，因此具有宝贵的教育和研究功能。

With National Science Foundation support, Dr. Todd Disotell and his collaborators at New York University will purchase an ABI PRISM 377 system which consists of an electrophoresis and detection unit, a Power Macintosh computer and software for DNA sequencing analysis or PCR fragment sizing and quantification. The system will allow high throughput through the use of multicolored fluorescence technology, allowing multiplex electrophoreses by co-loading the products of multiple PCR or sequencing reactions in the same lane. To increase the efficiency of the group of utilizing scientists, a thermocycler (Perkin-Elmer Geneamp PCR System 9700) along with a microcentrifuge (Eppendorf 5400 Series) and microconcentrator (Savant DNA SpeedVac) will be acquired and housed with the ABI genetic analyzer. A Power Macintosh computer will allow data collected on the ABI genetic analyzer to be analyzed off-line, allowing the computer controlling the ABI machine to be dedicated to data collection. This instrumentation will greatly improve the efficiency of six research projects, pursued by a number of investigators. These include the study of 1. the genetic and developmental mechanisms of morphogenesis and the evolution of form in rhabditid nematodes; 2. genetics of organogenesis in the gonad of C. elegans; 3.primate molecular evolution and population genetics; 4. evolutionary genetics of cave adaptations in fish; unidentified proteins in fission yeast that interact with Ras G-proteins and microtubules; and 6. recognition of DNA structure by HMG proteins. The machine will also be used at both graduate and undergraduate levels and thus serve a valuable educational as well as research function.