Interdepartmental (Biology, Anthropology, Chemistry) Genetic Analysis Facility

跨部门（生物学、人类学、化学）遗传分析设施

• 批准号: 9870970
• 负责人: Todd Disotell
• 金额: $ 11.5万
• 依托单位: New York University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9870970&HistoricalAwards=false
• 关键词: Interdepartmental; Biology; Anthropology; Chemistry; Genetic

With National Science Foundation support, Dr. Todd Disotell and his collaborators at New York University will purchase an ABI PRISM 377 system which consists of an electrophoresis and detection unit, a Power Macintosh computer and software for DNA sequencing analysis or PCR fragment sizing and quantification. The system will allow high throughput through the use of multicolored fluorescence technology, allowing multiplex electrophoreses by co-loading the products of multiple PCR or sequencing reactions in the same lane. To increase the efficiency of the group of utilizing scientists, a thermocycler (Perkin-Elmer Geneamp PCR System 9700) along with a microcentrifuge (Eppendorf 5400 Series) and microconcentrator (Savant DNA SpeedVac) will be acquired and housed with the ABI genetic analyzer. A Power Macintosh computer will allow data collected on the ABI genetic analyzer to be analyzed off-line, allowing the computer controlling the ABI machine to be dedicated to data collection. This instrumentation will greatly improve the efficiency of six research projects, pursued by a number of investigators. These include the study of 1. the genetic and developmental mechanisms of morphogenesis and the evolution of form in rhabditid nematodes; 2. genetics of organogenesis in the gonad of C. elegans; 3.primate molecular evolution and population genetics; 4. evolutionary genetics of cave adaptations in fish; unidentified proteins in fission yeast that interact with Ras G-proteins and microtubules; and 6. recognition of DNA structure by HMG proteins. The machine will also be used at both graduate and undergraduate levels and thus serve a valuable educational as well as research function.

在国家科学基金会的支持下，Todd Disotell 博士和他在纽约大学的合作者将购买 ABI PRISM 377 系统，该系统由电泳和检测装置、Power Macintosh 计算机以及用于 DNA 测序分析或 PCR 片段大小测定和定量的软件组成。该系统将通过使用多色荧光技术实现高通量，通过在同一泳道中共同加载多个 PCR 或测序反应的产物来实现多重电泳。为了提高科学家团队的效率，将购买一台热循环仪（Perkin-Elmer Geneamp PCR System 9700）、一台微量离心机（Eppendorf 5400 系列）和微浓缩器（Savant DNA SpeedVac），并与 ABI 基因分析仪一起安装。 Power Macintosh 计算机将允许对 ABI 基因分析仪上收集的数据进行离线分析，从而允许控制 ABI 机器的计算机专门用于数据收集。 该仪器将极大地提高许多研究人员所追求的六个研究项目的效率。这些包括： 1. 棒状线虫形态发生和形态进化的遗传和发育机制； 2. 秀丽隐杆线虫性腺器官发生的遗传学； 3.灵长类分子进化与群体遗传学； 4. 鱼类适应洞穴的进化遗传学；裂殖酵母中与 Ras G 蛋白和微管相互作用的未知蛋白质； 6. HMG蛋白对DNA结构的识别。该机器还将用于研究生和本科生，从而发挥有价值的教育和研究功能。