Interdepartmental (Biology, Anthropology, Chemistry) Genetic Analysis Facility

跨部门（生物学、人类学、化学）遗传分析设施

• 批准号: 9870970
• 负责人: Todd Disotell
• 金额: $ 11.5万
• 依托单位: New York University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9870970&HistoricalAwards=false
• 关键词: Interdepartmental; Biology; Anthropology; Chemistry; Genetic

With National Science Foundation support, Dr. Todd Disotell and his collaborators at New York University will purchase an ABI PRISM 377 system which consists of an electrophoresis and detection unit, a Power Macintosh computer and software for DNA sequencing analysis or PCR fragment sizing and quantification. The system will allow high throughput through the use of multicolored fluorescence technology, allowing multiplex electrophoreses by co-loading the products of multiple PCR or sequencing reactions in the same lane. To increase the efficiency of the group of utilizing scientists, a thermocycler (Perkin-Elmer Geneamp PCR System 9700) along with a microcentrifuge (Eppendorf 5400 Series) and microconcentrator (Savant DNA SpeedVac) will be acquired and housed with the ABI genetic analyzer. A Power Macintosh computer will allow data collected on the ABI genetic analyzer to be analyzed off-line, allowing the computer controlling the ABI machine to be dedicated to data collection. This instrumentation will greatly improve the efficiency of six research projects, pursued by a number of investigators. These include the study of 1. the genetic and developmental mechanisms of morphogenesis and the evolution of form in rhabditid nematodes; 2. genetics of organogenesis in the gonad of C. elegans; 3.primate molecular evolution and population genetics; 4. evolutionary genetics of cave adaptations in fish; unidentified proteins in fission yeast that interact with Ras G-proteins and microtubules; and 6. recognition of DNA structure by HMG proteins. The machine will also be used at both graduate and undergraduate levels and thus serve a valuable educational as well as research function.

在国家科学基金会的支持下，托德·迪索尔（Todd DiSotell）博士及其在纽约大学的合作者将购买ABI PRISM 377系统，该系统由电泳和检测单元组成，用于DNA测序分析或PCR片段尺寸和量化的Power Macintosh计算机和软件。该系统将通过使用多色荧光技术允许高通量，从而通过在同一泳道中共同加载多重PCR或测序反应的产物来允许多路复用电泳。为了提高利用科学家的效率，将使用ABI遗传分析仪（ABI Genetic Analialzer获取热中心（Perkin-Elmer GeneAmp PCR系统9700）以及微型离心机（Eppendorf 5400序列）和微浓度（Savant DNA Speedvac）。 Macintosh计算机将允许在ABI遗传分析仪上收集的数据离线分析，从而使控制ABI机器的计算机专用于数据收集。 该仪器将大大提高许多调查人员追求的六个研究项目的效率。其中包括对1的研究。形态发生的遗传和发育机制以及色丁氏菌线虫中形式的演变； 2。秀丽隐杆线虫性腺中器官发生的遗传学； 3.高级分子进化和种群遗传学； 4。鱼类洞穴适应的进化遗传学；与Ras G蛋白和微管相互作用的裂变酵母中未鉴定的蛋白质；和6。识别HMG蛋白对DNA结构的识别。该机器还将在研究生和本科水平上使用，从而提供有价值的教育和研究功能。