Equipment for Analysis Of Expressed Sequences In Diverse Species

该提案要求为购买设备提供资金，这些设备将使德克萨斯农工大学的不同研究人员受益。所需测序设备包括：Perkin Elmer 377-01 ABI Prism自动DNA测序仪和安装板；现有373A型自动DNA测序仪和安装板的Perkin Elmer拉伸升级；用于现有BIOMEK 2000机器人的Beckman 384针工具和安培套件；以及HP Deskjet 855CXi彩色打印机。对于下游分析，需要以下成像和数据记录设备：一台蔡司Axioplan 2显微镜；一台Meyer Instruments彩色制冷3片CCD摄像机和成像系统；用于PC&Amp；Mac和一台奔腾P586/166计算机的CCD摄像机软件、固件和连接计算机硬件。请提供所列设备，以便利生物系几名成员进行的动物、植物和微生物科学研究。此外，基因技术实验室(GTL)是发育和分子生物学研究所(IDMB)内的一个服务实体，它的设施对德克萨斯农工大学社区的所有成员都是可用的。由于现有DNA测序设施的使用已经达到饱和水平，除非获得更多的设备，否则将禁止对指定研究人员和其他人的研究。人们普遍需要确定许多分离基因的功能。我们目前的成像设施只能提供有限的资源来满足这一需求，购买所需的设备将是朝着使IDMB能够为下游分析提供服务的重要一步，这将补充目前的测序服务。所要求的物品是比个别国际组织实验室通常能够获得的更复杂的设备，并将大大提高数据收集和报告的质量。A-1

This proposal requests funds to purchase equipment that will benefit a diverse group of researchers at Texas A&M University. Sequencing equipment requested consists of: a Perkin Elmer 377-01 ABI Prism Automated DNA Sequencer & Plates; a Perkin Elmer Stretch Upgrade for the existing Model 373A Automated Sequencer & Plates; a Beckman 384-Pin Tool & Kit for an existing BioMek 2000 robot, and an HP Deskjet 855CXi Color Printer. For downstream analysis, the following equipment for imaging and data recording is requested: a Zeiss Axioplan 2 Microscope; a Meyer Instruments Color Chilled 3-Chip CCD Camera and Imaging System; CCD Camera software, firmware and connective computer hardware for PC & Mac and a Pentium P586/166 Computer to run the CCD camera. The listed equipment is requested to facilitate research in animal, plant and microbial science undertaken by several members of the Department of Biology. Additionally, the facilities of the Gene Technologies Laboratory (GTL) which is a service entity within the Institute of Developmental and Molecular Biology (IDMB), is available to all members of the Texas A&M University community. Since the use of the available DNA sequencing facilities has reached saturation level, studies of the named researchers, and others, will be inhibited unless additional equipment is acquired. There is a widespread need to characterize the functionality of many of the isolated genes. Our present imaging facilities provide only limited resources towards this need and acquisition of the requested equipment would represent an important step towards enabling the IDMB to provide services for downstream analysis that will complement those of the present sequencing service. The requested items represent more sophisticated equipment than can usually be acquired by an individual PI's laboratory, and will greatly enhance the quality of data gathering and reporting. A - 1