Equipment for Analysis Of Expressed Sequences In Diverse Species

这项提案要求资金购买设备，这将有利于在得克萨斯州A M大学的研究人员的多样化群体。 申请的测序设备包括：Perkin Elmer 377-01 ABI Prism自动DNA测序仪板;现有373 A型自动测序仪板的Perkin Elmer Stretch升级版;现有BioMek 2000机器人的Beckman 384针工具包和HP Deskjet 855 CXi彩色打印机。对于下游分析，需要以下用于成像和数据记录的设备：Zeiss Axioplan 2显微镜; Meyer Instruments彩色冷却3芯片CCD照相机和成像系统; CCD照相机软件、固件和用于PC Mac的连接计算机硬件以及运行CCD照相机的Pentium P586/166计算机。 请求提供所列设备是为了便利生物系几名成员进行的动物、植物和微生物科学研究。此外，基因技术实验室（GTL）是发育和分子生物学研究所（IDMB）内的服务实体，其设施可供德克萨斯州A M大学社区的所有成员使用。由于现有DNA测序设施的使用已达到饱和水平，除非获得额外的设备，否则将禁止指定研究人员和其他人的研究。有一个广泛的需求，以表征许多孤立的基因的功能。我们目前的成像设施只能提供有限的资源来满足这一需求，获得所需的设备将是使IDMB能够提供下游分析服务的重要一步，这将补充目前的测序服务。所要求的物品是比个别主要研究者实验室通常所能获得的更先进的设备，将大大提高数据收集和报告的质量。 A - 1

This proposal requests funds to purchase equipment that will benefit a diverse group of researchers at Texas A&M University. Sequencing equipment requested consists of: a Perkin Elmer 377-01 ABI Prism Automated DNA Sequencer & Plates; a Perkin Elmer Stretch Upgrade for the existing Model 373A Automated Sequencer & Plates; a Beckman 384-Pin Tool & Kit for an existing BioMek 2000 robot, and an HP Deskjet 855CXi Color Printer. For downstream analysis, the following equipment for imaging and data recording is requested: a Zeiss Axioplan 2 Microscope; a Meyer Instruments Color Chilled 3-Chip CCD Camera and Imaging System; CCD Camera software, firmware and connective computer hardware for PC & Mac and a Pentium P586/166 Computer to run the CCD camera. The listed equipment is requested to facilitate research in animal, plant and microbial science undertaken by several members of the Department of Biology. Additionally, the facilities of the Gene Technologies Laboratory (GTL) which is a service entity within the Institute of Developmental and Molecular Biology (IDMB), is available to all members of the Texas A&M University community. Since the use of the available DNA sequencing facilities has reached saturation level, studies of the named researchers, and others, will be inhibited unless additional equipment is acquired. There is a widespread need to characterize the functionality of many of the isolated genes. Our present imaging facilities provide only limited resources towards this need and acquisition of the requested equipment would represent an important step towards enabling the IDMB to provide services for downstream analysis that will complement those of the present sequencing service. The requested items represent more sophisticated equipment than can usually be acquired by an individual PI's laboratory, and will greatly enhance the quality of data gathering and reporting. A - 1