Equipment for Analysis Of Expressed Sequences In Diverse Species

该提案要求拨款购买设备，使德克萨斯农工大学的各种研究人员受益。所要求的测序设备包括：Perkin Elmer 377-01 ABI Prism自动DNA测序仪；珀金埃尔默拉伸升级现有的373A型自动测序仪；一个贝克曼384针工具套件，用于现有的BioMek 2000机器人，以及一台惠普Deskjet 855CXi彩色打印机。对于下游分析，需要以下成像和数据记录设备：蔡司Axioplan 2显微镜；Meyer Instruments彩色冷冻三芯片CCD相机及成像系统用于PC和Mac的CCD相机软件、固件和连接的计算机硬件，以及运行CCD相机的奔腾P586/166计算机。所列设备是为了促进生物系若干成员进行的动物、植物和微生物科学研究。此外，基因技术实验室（GTL）的设施是发育和分子生物学研究所（IDMB）的一个服务实体，对德克萨斯农工大学社区的所有成员开放。由于现有DNA测序设备的使用已经达到饱和水平，上述研究人员和其他人的研究将被禁止，除非获得额外的设备。人们普遍需要对许多分离基因的功能进行表征。我们目前的成像设备只能提供有限的资源来满足这一需求，获得所要求的设备将是使IDMB能够提供下游分析服务的重要一步，这将补充现有的测序服务。所要求的项目代表着比单个PI实验室通常能够获得的更复杂的设备，并将大大提高数据收集和报告的质量。A - 1

This proposal requests funds to purchase equipment that will benefit a diverse group of researchers at Texas A&M University. Sequencing equipment requested consists of: a Perkin Elmer 377-01 ABI Prism Automated DNA Sequencer & Plates; a Perkin Elmer Stretch Upgrade for the existing Model 373A Automated Sequencer & Plates; a Beckman 384-Pin Tool & Kit for an existing BioMek 2000 robot, and an HP Deskjet 855CXi Color Printer. For downstream analysis, the following equipment for imaging and data recording is requested: a Zeiss Axioplan 2 Microscope; a Meyer Instruments Color Chilled 3-Chip CCD Camera and Imaging System; CCD Camera software, firmware and connective computer hardware for PC & Mac and a Pentium P586/166 Computer to run the CCD camera. The listed equipment is requested to facilitate research in animal, plant and microbial science undertaken by several members of the Department of Biology. Additionally, the facilities of the Gene Technologies Laboratory (GTL) which is a service entity within the Institute of Developmental and Molecular Biology (IDMB), is available to all members of the Texas A&M University community. Since the use of the available DNA sequencing facilities has reached saturation level, studies of the named researchers, and others, will be inhibited unless additional equipment is acquired. There is a widespread need to characterize the functionality of many of the isolated genes. Our present imaging facilities provide only limited resources towards this need and acquisition of the requested equipment would represent an important step towards enabling the IDMB to provide services for downstream analysis that will complement those of the present sequencing service. The requested items represent more sophisticated equipment than can usually be acquired by an individual PI's laboratory, and will greatly enhance the quality of data gathering and reporting. A - 1