Functional analysis of long noncoding RNAs expressed in the brain

大脑中表达的长非编码RNA的功能分析

• 批准号: DP210103233
• 负责人: Prof John Mattick
• 金额: $ 45.7万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-06-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP210103233
• 关键词: Functional; analysis; long; noncoding; RNAs

For many years, the mammalian genome has been thought to be mainly junk. Recently, however, it has become evident that most of the genome specifies RNAs that do not encode proteins ('long noncoding' RNAs, lncRNAs), many of which are brain-specific. This project aims to determine the functions of lncRNAs that are expressed in the hippocampus (involved in learning) and the cerebellum (involved in movement coordination) by deleting them in mice, testing for developmental, cognitive and motor effects, and characterising the structures with which they are associated. The results of the project are expected to open new vistas in neuroscience, contributing to understanding the molecular basis of brain function and the 'dark matter' of the genome.

多年来，哺乳动物基因组一直被认为主要是垃圾。然而，最近，很明显，大多数基因组指定不编码蛋白质的RNA（“长非编码”RNA，lncRNA），其中许多是大脑特异性的。该项目旨在通过在小鼠中删除海马体（参与学习）和小脑（参与运动协调）中表达的lncRNA，测试其发育、认知和运动效应，并表征与其相关的结构，从而确定这些lncRNA的功能。该项目的结果预计将为神经科学开辟新的前景，有助于理解大脑功能的分子基础和基因组的“暗物质”。