Confocal Microscope for Cellular, Molecular and Neural Biology

需要资金购买徕卡TCS NT共焦显微镜系统，以建立多用户共焦设施。这一系统最适合用户群体的需要以及这里提出的不同项目和方法。用户组代表Salk教职员工和部门代表的一个横截面。所要求的共焦系统的11个列出的用户包括索尔克研究所5个系的教职员工代表。7个主要用户包括4个系的教职员工代表，列出的4个次要用户包括3个系的教职员工代表。该建议中包含的研究项目强调用细胞和分子方法研究实验生物学中的广泛问题，包括染色体复制和分离、DNA复制的调节、大分子从细胞核到细胞质的运输、神经递质受体和离子通道在大脑中的定位，以及神经发育中的基本事件，如神经诱导、细胞命运和分化、神经元迁移和轴突引导，以及神经回路的发展。

Funds are requested to purchase a Leica TCS NT confocal microscope system to establish a multiple user confocal facility. This system best fits the needs of the user group and the diverse projects and approaches proposed here. The user group represents a cross section of the Salk faculty and departmental representation. The11 listed users of the requested confocal system include faculty representatives of 5 departments of the Salk Institute. The 7 major users include faculty representatives of 4 departments, and the 4 minor users listed include faculty representatives of 3 departments. The research projects contained within this proposal emphasize cellular and molecular approaches to study a broad range of issues in experimental biology, including chromosome replication and segregation, regulators of DNA replication, the trafficking of macromolecules from the nucleus to the cytoplasm, localization of neurotransmitter receptors and ion channel in the brain, and fundamental events in neural development such as neural induction, cell fate and differentiation, neuronal migration and axon guidance, and the development of neural circuits.