Joint 3-D Reconstruction from Cryo Electron Microscopy and Solution X-ray Scattering Data

利用冷冻电子显微镜和溶液 X 射线散射数据进行联合 3D 重建

• 批准号: 9630497
• 负责人: Peter Doerschuk
• 金额: $ 17.61万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-15 至 2001-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9630497&HistoricalAwards=false
• 关键词: Joint; Reconstruction; Cryo; Electron; Microscopy

The objective of the research is the development of new methods for jointly analyzing cryoelectron microscopy (EM) and solution x-ray scattering data in order to determine the 3D structure of spherical viruses. Research in this area is timely because improved microscopes and cryo EM techniques are increasing the flow of images while synchrotrons and associated low-angle scattering beam lines allow the measurement of qualitatively superior solution x-ray scattering data. The joint use of both types of data is a natural approach because of compensating strengths and weaknesses. For instance, cryo EM images depend on unknown orientation parameters while solution x-ray scattering data does not. There are two major themes that extend through the work: a new model for the scattering density of the particle and a unified way of combining the model with all of the experimental data in order to reconstruct the particle. The purpose of the model is to precisely and concisely describe the particle. In particular, the proposed model enforces the finite real-space extent of the particle, allows more than one type of symmetry to exist with an individual particle, has few parameters, and is mathematically simple in both real and reciprocal space.

该研究的目的是开发联合分析低温电子显微镜（EM）和溶液x射线散射数据的新方法，以确定球形病毒的三维结构。这一领域的研究是及时的，因为改进的显微镜和低温电镜技术正在增加图像的流量，而同步加速器和相关的低角度散射光束线允许测量质量优越的溶液x射线散射数据。联合使用这两种类型的数据是一种自然的方法，因为它们可以弥补各自的优缺点。例如，冷冻电镜图像依赖于未知的方向参数，而溶液x射线散射数据则不依赖于未知的方向参数。有两个主要的主题延伸到整个工作：一个新的粒子散射密度模型和一个统一的方法，将模型与所有的实验数据相结合，以重建粒子。该模型的目的是精确而简洁地描述粒子。特别是，所提出的模型强化了粒子的有限实空间范围，允许单个粒子存在不止一种类型的对称性，具有很少的参数，并且在实空间和倒数空间中都具有数学上的简单性。