ITR/AP (BIO) Computational tools for determining the 3-D static and dynamic structure of viruses

ITR/AP (BIO) 用于确定病毒 3D 静态和动态结构的计算工具

• 批准号: 0112672
• 负责人: Peter Doerschuk
• 金额: $ 35.93万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-10-01 至 2006-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0112672&HistoricalAwards=false
• 关键词: ITR; AP; BIO; Computational; tools

ITR Proposal 0112672, DoerschukAbstractUnderstanding the dynamical behavior of virus particles, e.g., the self assembly and subsequent maturation steps that lead to an infectious virus particle, is a key challenge in basic biology (e.g., the understanding of protein-nucleic acid interactions) and in medicine (e.g., the development of drugs that interfere with viral replication). This research contributes to that goal by developing new computational tools for structural biology, emphasizing experimental approaches appropriate for the study of dynamics and emphasizing the bi-directional interplay of new structural biology problems and new formulations of existing structural biology problems with the development of new computational tools.Models and computation for new types of experiments are under development, e.g., x-ray scattering from solutions of labeled viral particles which are oriented by immersion in a strong electric field thereby transforming solution scattering to something analogous to the more informative fiber diffraction. New computational approaches to standard problems are also under development, e.g., phase retrieval for the x-ray crystallography of particles with non-crystallographic symmetries based on iterative phase retrieval algorithms applied to lattices that are computationally oversampled by interpolators based on the symmetry. Computational areas involved include fast 3-D Radon transform algorithms, a component of computing the cryo electron micrograph predicted from a given virus structure, and global optimization, in order to improve the values of the parameters that describe the virus structure.The PIs have a long term interest in undergraduate involvement in research and financial support for undergraduate research assistants is included.

ITR提案0112672，Doerschuk摘要了解病毒粒子的动力学行为，例如，导致感染性病毒颗粒的自组装和随后的成熟步骤，是基础生物学中的关键挑战（例如，对蛋白质-核酸相互作用的理解）和医学（例如，开发干扰病毒复制的药物）。 该研究通过开发新的结构生物学计算工具，强调适合于动力学研究的实验方法，并强调新的结构生物学问题和现有结构生物学问题的新公式与新的计算工具的发展的双向相互作用，为这一目标做出了贡献。来自标记的病毒颗粒溶液的X射线散射，所述病毒颗粒通过浸没在强电场中而定向，从而将溶液散射转化为类似于更有信息的纤维衍射的东西。 标准问题的新计算方法也在开发中，例如，用于具有非晶体对称性的粒子的X射线晶体学的相位恢复，该相位恢复基于迭代相位恢复算法，该迭代相位恢复算法应用于通过基于对称性的插值器计算过采样的晶格。 所涉及的计算领域包括快速3-D Radon变换算法，从一个给定的病毒结构预测的低温电子显微图计算的一个组成部分，和全局优化，以提高描述病毒结构的参数的值。PI对本科生参与研究和本科生研究助理的财政支持有着长期的兴趣。