Development of a Molecular Biology Track Focused on the Population Genetic Analysis of Threatened and Endangered Species

发展以受威胁和濒危物种群体遗传分析为重点的分子生物学方向

• 批准号: 9650319
• 负责人: Lauretta Bushar
• 金额: $ 5.84万
• 依托单位: Arcadia University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9650319&HistoricalAwards=false
• 关键词: Development; Molecular; Biology; Track; Focused

The purpose of this project is the development of a curriculum in cell and molecular biology with a laboratory focusing on the population genetic analysis of threatened and endangered species. This can be an integral part of the Biology major. It fits into the curriculum well because the research problems being addressed combine molecular techniques with an application that has intrinsic relevance to students with a strong interest in organismal biology. The plan is to accomplish this objective through the distribution of basic concepts and techniques among several courses at the introductory (100 and 200) level followed by the application of these techniques in upper-level (300) courses. The focus of the laboratory work in the upper-level courses is on the development and usage of microsatellite genetic markers designed specifically for population genetic analysis of a particular threatened or endangered species. As a result of this program, students are gaining experience with molecular techniques such as DNA isolation, restriction analysis, Southern blotting, cloning, library construction and screening, DNA sequencing, and polymerase chain reactions. Audiences targeted by this program include women, who comprise 75 percent of our student population, prospective teachers (about 35 per year), and biology majors. The impact on curricular development can be analyzed by surveys and course evaluations. It is further expected that the laboratory activities developed in this program will be published in journals and in laboratory manuals.

该项目的目的是开发细胞和分子生物学课程，并建立一个实验室，重点研究受威胁和濒危物种的种群遗传分析。这可以成为生物学专业的一个组成部分。它很好地融入了课程，因为所要解决的研究问题将分子技术与对有机生物学有浓厚兴趣的学生具有内在相关性的应用相结合。计划是通过在入门级（100和200）的几门课程中分配基本概念和技术，然后在高级（300）课程中应用这些技术来实现这一目标。高级课程实验室工作的重点是发展和使用微卫星遗传标记，这些标记是专门为某一受威胁或濒危物种的种群遗传分析而设计的。由于这个项目，学生们获得了分子技术的经验，如DNA分离，限制分析，南方印迹，克隆，文库建设和筛选，DNA测序和聚合酶链反应。这个项目的目标受众包括女性，她们占我们学生总数的75%，未来的教师（每年约35人）和生物学专业的学生。对课程发展的影响可以通过调查和课程评价来分析。进一步期望在该计划中开发的实验室活动将发表在期刊和实验室手册中。