Template Polymerized Chelating Liposomes for Separation and Recovery of Biomolecules from Aqueous Solutions

摘要建议编号：9708007建议类型：研究人员发起的主要研究人员：罗伯托·F·古兹曼，大卫·F·奥布莱恩隶属：亚利桑那大学这笔赠款是通过化学和运输系统司界面、运输和分离计划的分离和净化计划子要素授予的。主要研究人员是亚利桑那大学的罗伯托·F·古兹曼和大卫·F·奥布莱恩。本研究探索了一种结合分子印迹、固定化金属离子亲和层析和表面修饰模板金属离子螯合剂修饰的高比表面积单层脂质体的优点和独特的选择性的蛋白质纯化方法的可行性。该方法旨在提供从复杂介质中高产率、选择性和保持天然蛋白质构象的纯化蛋白质的手段。该方法是金属离子亲和层析的一种高度选择性的使用，有可能被开发为具有多个结合位点的生物分子的一种有效的纯化方案。生物活性大分子的生产，如诊断酶、抗体、激素等，是生物技术的核心。一个主要的问题是这些生物分子的纯化和生物活性的保留。这里将要开发的技术有可能解决这些问题。

Abstract Proposal No: 9708007 Proposal Type: Investigator Initiated Principal Investigators: Roberto F. Guzman, David F. O'Brien Affiliation: University of Arizona This grant is awarded through the Separations and Purification Program sub-element of the Interfacial, Transport and Separations Program of the Chemical and Transport Systems Division. The principal investigators are Roberto F. Guzman and David F. O'Brien of the University of Arizona. The research explores the feasibility of a method of protein purification that combines the advantages and unique selectivity of molecular imprinting, immobilized metal ion affinity chromatography, and the high surface area of unilamellar liposomes modified with surface associated templated metal ion-chelators. The method aims to provide the means to purify proteins from complex media with high yields, selectivity and maintenance of native protein conformation. The method is a highly selective use of metal ion affinity chromatorgraphy that has the potential to be developed as an efficient purification scheme for biomolecules with multiple binding sites. The production of bioactive macromolecules such as diagnostic enzymes, antibodies, hormones etc., is central to biotechnology. A major issue is the purification of these biomolecules with retention of bioactivity. The technology to be developed here has the potential to address these issues.