Template Polymerized Chelating Liposomes for Separation and Recovery of Biomolecules from Aqueous Solutions

用于从水溶液中分离和回收生物分子的模板聚合螯合脂质体

• 批准号: 9708007
• 负责人: Roberto Guzman
• 金额: $ 19万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-01 至 2001-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9708007&HistoricalAwards=false
• 关键词: Template; Polymerized; Chelating; Liposomes; Separation

Abstract Proposal No: 9708007 Proposal Type: Investigator Initiated Principal Investigators: Roberto F. Guzman, David F. O'Brien Affiliation: University of Arizona This grant is awarded through the Separations and Purification Program sub-element of the Interfacial, Transport and Separations Program of the Chemical and Transport Systems Division. The principal investigators are Roberto F. Guzman and David F. O'Brien of the University of Arizona. The research explores the feasibility of a method of protein purification that combines the advantages and unique selectivity of molecular imprinting, immobilized metal ion affinity chromatography, and the high surface area of unilamellar liposomes modified with surface associated templated metal ion-chelators. The method aims to provide the means to purify proteins from complex media with high yields, selectivity and maintenance of native protein conformation. The method is a highly selective use of metal ion affinity chromatorgraphy that has the potential to be developed as an efficient purification scheme for biomolecules with multiple binding sites. The production of bioactive macromolecules such as diagnostic enzymes, antibodies, hormones etc., is central to biotechnology. A major issue is the purification of these biomolecules with retention of bioactivity. The technology to be developed here has the potential to address these issues.

摘要 提案编号：9708007 提案类型：研究者发起 主要研究者：Roberto F. Guzman、David F. O'Brien 隶属关系：亚利桑那大学 这笔赠款是通过化学和运输系统部门界面、运输和分离计划的分离和纯化计划子项目授予的。主要研究人员是亚利桑那大学的 Roberto F. Guzman 和 David F. O'Brien。该研究探索了一种蛋白质纯化方法的可行性，该方法结合了分子印迹、固定金属离子亲和色谱的优点和独特选择性，以及表面相关模板金属离子螯合剂修饰的单层脂质体的高表面积。该方法旨在提供从复杂介质中纯化蛋白质的方法，具有高产量、高选择性和维持天然蛋白质构象的优点。该方法是对金属离子亲和色谱的高度选择性使用，有潜力开发为具有多个结合位点的生物分子的有效纯化方案。 诊断酶、抗体、激素等生物活性大分子的生产是生物技术的核心。一个主要问题是纯化这些生物分子并保留生物活性。这里要开发的技术有可能解决这些问题。