Acquisition of a Fluorescence Spectrometer for Research in Protein Catalysis and Folding

*9709164诺贝尔化学研究仪器和设施计划颁发的这一奖项将帮助位于斯通布鲁克的纽约州立大学化学系获得一台荧光光谱仪。该设备将加强以下领域的研究：(1)结合和荧光猝灭实验，以探测蛋白质的部分折叠状态；(2)蛋白质表面环在界面酶胆固醇氧化酶催化的反应中的作用；(3)Enoyl-CoA水合酶和Acyl-CoA脱氢酶的作用机制。荧光光谱仪计算紫外光和可见光区域的光子数，当材料样品被紫外光照射时，过滤以吸收反射的紫外光，然后只检测到可见荧光。该光谱仪用于快速发生的荧光猝灭实验，并将允许在不同温度下使用驰豫过程。***

*** 9709164 LE NOBLE This award from the Chemistry Research Instrumentation and Facilities (CRIF) Program will help the Department of Chemistry at the SUNY at Stony Brook to acquire a fluorescence spectrometer. This equipment will enhance research in a number of areas including the following: (1) binding and fluorescence quenching experiments to probe partially folded states of proteins, (2) the role of protein surface loops in the reaction catalyzed by the interfacial enzyme cholesterol oxidase, and (3) the mechanism of the enzymes enoyl-CoA hydratase and acyl-CoA dehydrogenase. The fluorescence spectrometer counts photons of light in the UV and visible regions and when a sample of material is illuminated by UV light, filtered to absorb the reflected UV light, then only visible fluorescence is detected. The spectrometer is used in fluorescence quenching experiments which occur at fast rates, and will allow relaxation processes to be used at various temperatures. ***