Acquisition of a Fluorescence Spectrometer for Research in Protein Catalysis and Folding

购买荧光光谱仪用于蛋白质催化和折叠研究

• 批准号: 9709164
• 负责人: Iwao Ojima
• 金额: $ 4.39万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9709164&HistoricalAwards=false
• 关键词: Acquisition; Fluorescence; Spectrometer; Research; Protein

*** 9709164 LE NOBLE This award from the Chemistry Research Instrumentation and Facilities (CRIF) Program will help the Department of Chemistry at the SUNY at Stony Brook to acquire a fluorescence spectrometer. This equipment will enhance research in a number of areas including the following: (1) binding and fluorescence quenching experiments to probe partially folded states of proteins, (2) the role of protein surface loops in the reaction catalyzed by the interfacial enzyme cholesterol oxidase, and (3) the mechanism of the enzymes enoyl-CoA hydratase and acyl-CoA dehydrogenase. The fluorescence spectrometer counts photons of light in the UV and visible regions and when a sample of material is illuminated by UV light, filtered to absorb the reflected UV light, then only visible fluorescence is detected. The spectrometer is used in fluorescence quenching experiments which occur at fast rates, and will allow relaxation processes to be used at various temperatures. ***

* 9709164 LE NOBLE化学研究仪器和设施奖 （CRIF）计划将帮助纽约州立大学斯托尼分校化学系 布鲁克去弄一个荧光光谱仪。该设备将增强 在一些领域的研究，包括以下几个方面：（1）约束力和 荧光猝灭实验，以探测部分折叠状态， 蛋白质，（2）蛋白质表面环在催化反应中的作用 界面酶胆固醇氧化酶，和（3）的机制， 烯酰辅酶A水合酶和酰基辅酶A脱氢酶。 荧光光谱仪对紫外光中的光子进行计数， 可见光区域和材料样品被UV照射时 光，过滤吸收反射的紫外光，然后只可见 检测荧光。光谱仪用于荧光 淬火实验发生在快速，并将允许 在不同温度下使用的弛豫过程。 ***