Acquisition of a Fluorescence Spectrometer for Research in Protein Catalysis and Folding

来自化学研究仪器和设施（CRIF）计划的这项奖励将帮助纽约州立大学石溪分校化学系获得一台荧光光谱仪。该设备将加强多个领域的研究，包括：(1)结合和荧光猝灭实验，探测蛋白质的部分折叠状态；(2)蛋白质表面环在界面酶胆固醇氧化酶催化的反应中的作用；(3)烯酰辅酶a水合酶和酰基辅酶a脱氢酶的作用机制。荧光光谱仪计数紫外线和可见光区的光子，当材料样品被紫外线照射，过滤以吸收反射的紫外线，然后只检测到可见的荧光。该光谱仪用于快速荧光猝灭实验，并允许在不同温度下使用弛豫过程。＊＊＊

*** 9709164 LE NOBLE This award from the Chemistry Research Instrumentation and Facilities (CRIF) Program will help the Department of Chemistry at the SUNY at Stony Brook to acquire a fluorescence spectrometer. This equipment will enhance research in a number of areas including the following: (1) binding and fluorescence quenching experiments to probe partially folded states of proteins, (2) the role of protein surface loops in the reaction catalyzed by the interfacial enzyme cholesterol oxidase, and (3) the mechanism of the enzymes enoyl-CoA hydratase and acyl-CoA dehydrogenase. The fluorescence spectrometer counts photons of light in the UV and visible regions and when a sample of material is illuminated by UV light, filtered to absorb the reflected UV light, then only visible fluorescence is detected. The spectrometer is used in fluorescence quenching experiments which occur at fast rates, and will allow relaxation processes to be used at various temperatures. ***