Protein Transport and Chromatography with Gel-Composite Ion-Exchangers

提案编号：9709670提案类型：研究者发起首席研究员：Giorgio Carta所属机构：弗吉尼亚大学该基金通过化学与运输系统部界面、传输与分离项目的分离与净化项目子项目授予。首席研究员是弗吉尼亚大学的乔治·卡塔博士。该研究涉及凝胶复合离子交换器中蛋白质和其他大分子的扩散运输特性以及这些材料在蛋白质色谱中的应用。所述材料是通过在刚性支撑基质的孔隙内合成功能化聚合物凝胶而获得的，从而实现气孔的基本完全填充。因此，凝胶对机械力和溶液条件的变化是稳定的，允许在流动相的高流速下进行色谱操作。本研究探讨了工艺规模蛋白质色谱新技术的发展。对凝胶中蛋白质转运的基本理解将有助于开发新的蛋白质分离技术，特别是那些面向大容量快速分离的技术。

Abstract Proposal No: 9709670 Proposal Type: Investigator Initiated Principal Investigator: Giorgio Carta Affiliation: University of Virginia This grant is awarded through the Separations and Purification Program sub-element of the Interfacial, Transport and Separations Program of the Chemical and Transport Systems Division. The principal investigator is Dr. Giorgio Carta of the University of Virginia. The research is concerned with the characterization of diffusional transport of proteins and other macromolecules in gel-composite ion-exchangers and with the use of these materials in protein chromatography. The materials are obtained by synthesizing functionalized polymer gels within the pores of a rigid support matrix such that an essentially complete filling of the pores is achieved. The gel is thus stable to mechanical forces and to changes in solution conditions, allowing chromatographic operations at elevated flow rates of the mobile phase. This research addresses the development of new technologies for process scale protein chromatography. The fundamental understanding of protein transport in gels will aid development of new protein separation technologies, especially those oriented to high volume rapid separations.