Protein Transport and Chromatography with Gel-Composite Ion-Exchangers

摘要 提案编号： 9709670 提案类型： 研究者发起的主要研究者： Giorgio Carta附属机构： 弗吉尼亚大学 该补助金通过化学和运输系统司界面，运输和分离计划的分离和纯化计划子要素授予。首席研究员是弗吉尼亚大学的乔治·卡塔博士。本研究涉及蛋白质和其他大分子在凝胶复合离子交换剂中的扩散传输特性，以及这些材料在蛋白质色谱中的应用。所述材料通过在刚性载体基质的孔内合成官能化聚合物凝胶以实现孔的基本完全填充而获得。因此，凝胶对机械力和溶液条件的变化是稳定的，允许在移动的相的高流速下进行色谱操作。 本研究致力于工艺规模蛋白质色谱新技术的开发。对蛋白质在凝胶中转运的基本理解将有助于开发新的蛋白质分离技术，特别是那些面向大容量快速分离的技术。

Abstract Proposal No: 9709670 Proposal Type: Investigator Initiated Principal Investigator: Giorgio Carta Affiliation: University of Virginia This grant is awarded through the Separations and Purification Program sub-element of the Interfacial, Transport and Separations Program of the Chemical and Transport Systems Division. The principal investigator is Dr. Giorgio Carta of the University of Virginia. The research is concerned with the characterization of diffusional transport of proteins and other macromolecules in gel-composite ion-exchangers and with the use of these materials in protein chromatography. The materials are obtained by synthesizing functionalized polymer gels within the pores of a rigid support matrix such that an essentially complete filling of the pores is achieved. The gel is thus stable to mechanical forces and to changes in solution conditions, allowing chromatographic operations at elevated flow rates of the mobile phase. This research addresses the development of new technologies for process scale protein chromatography. The fundamental understanding of protein transport in gels will aid development of new protein separation technologies, especially those oriented to high volume rapid separations.