Protein Transport and Chromatography with Gel-Composite Ion-Exchangers

摘要建议编号：9709670建议类型：调查员发起的首席调查员：乔治·宪章隶属关系：弗吉尼亚大学这项拨款是通过化学和运输系统司界面、运输和分离计划中的分离和净化计划子要素授予的。首席研究员是弗吉尼亚大学的乔治·卡尔塔博士。这项研究涉及蛋白质和其他大分子在凝胶复合离子交换器中的扩散传输的表征，以及这些材料在蛋白质层析中的应用。该材料是通过在刚性支撑基质的孔内合成功能化的聚合物凝胶来获得的，从而实现了孔的基本完全填充。因此，凝胶对机械力和溶液条件的变化都是稳定的，允许在流动相流速较高的情况下进行色谱操作。这项研究致力于过程规模蛋白质层析新技术的开发。对蛋白质在凝胶中传输的基本了解将有助于发展新的蛋白质分离技术，特别是那些面向大容量快速分离的技术。

Abstract Proposal No: 9709670 Proposal Type: Investigator Initiated Principal Investigator: Giorgio Carta Affiliation: University of Virginia This grant is awarded through the Separations and Purification Program sub-element of the Interfacial, Transport and Separations Program of the Chemical and Transport Systems Division. The principal investigator is Dr. Giorgio Carta of the University of Virginia. The research is concerned with the characterization of diffusional transport of proteins and other macromolecules in gel-composite ion-exchangers and with the use of these materials in protein chromatography. The materials are obtained by synthesizing functionalized polymer gels within the pores of a rigid support matrix such that an essentially complete filling of the pores is achieved. The gel is thus stable to mechanical forces and to changes in solution conditions, allowing chromatographic operations at elevated flow rates of the mobile phase. This research addresses the development of new technologies for process scale protein chromatography. The fundamental understanding of protein transport in gels will aid development of new protein separation technologies, especially those oriented to high volume rapid separations.