Protein Transport in Charged Gels for Chromatography and Membrane Applications

用于色谱和膜应用的带电凝胶中的蛋白质运输

基本信息

  • 批准号:
    0414143
  • 负责人:
  • 金额:
    $ 27.7万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2004
  • 资助国家:
    美国
  • 起止时间:
    2004-08-15 至 2008-07-31
  • 项目状态:
    已结题

项目摘要

AbstractProposal Title: Protein Transport in Charged Gels for Chromatography and Membrane ApplicationsProposal Number: CTS-0414143Principal Investigator: Georgio CartaInstitution: University of VirginiaThe objective of this project is to develop a fundamental understanding of protein partitioning and transport in charged hydrogels. Understanding the transport properties of charged macromolecules in such materials is a key to their development and optimum design. New insights have recently been obtained through microscopic studies, which can provide the basis to develop a mechanistic understanding of transport. This project will expand current efforts in this area with the ultimate goal of developing new adsorbents and membranes for protein separations as well as models to predict their process performance. Protein partitioning and transport in charged dextran gels and agarose-dextran composites will be studied on a microscopic scale focusing on the effects of gel properties and protein molecular weight and charge on equilibrium uptake and mass transfer kinetics. Kinetics of protein ion-exchange in charged poly(acrylamide) gels and charged agarose-dextran composites will be studied using radiotracer techniques to elucidate protein transport under both gradient and tracer diffusion conditions. Modeling single and multicomponent protein transport in ionic gels and application of these models to predict the behavior of column chromatography under processing conditions will be performed. This research will provide a higher level of understanding of transport phenomena in these complex media as well as models to aid the design and optimization of chromatographic processes. The research will have multiple broader impacts. It will guide the development of optimized stationary phases by media manufacturers. It will also provide a basis for the formulation of rate models to predict and optimize chromatographic separations permitting pharmaceutical companies to improve the design and efficiency of bioprocesses. Since separation and purification are often the costliest steps in bioprocessing, there is a critical need for separation scientists and engineers who are trained to develop and implement novel separation media integrating a broad spectrum of experimental and modeling approaches to improve the effectiveness of industrial bioseparations.
摘要提案标题:色谱和膜应用中荷电凝胶中的蛋白质转运提案编号:CTS-0414143主要研究者:Georgio Carta机构: 该项目的目的是发展一个基本的了解蛋白质分配和运输的带电水凝胶。 了解带电大分子在这类材料中的输运性质是其开发和优化设计的关键。 最近通过微观研究获得了新的见解,这可以为发展对运输的机械理解提供基础。 该项目将扩大目前在这一领域的努力,最终目标是开发用于蛋白质分离的新吸附剂和膜,以及预测其过程性能的模型。 蛋白质分配和运输带电葡聚糖凝胶和琼脂糖-葡聚糖复合材料将在微观尺度上研究,重点是凝胶性质和蛋白质分子量和电荷对平衡吸收和传质动力学的影响。 将使用放射性示踪技术研究带电聚丙烯酰胺凝胶和带电琼脂糖-葡聚糖复合物中的蛋白质离子交换动力学,以阐明梯度和示踪扩散条件下的蛋白质转运。 将进行离子凝胶中单一和多组分蛋白质转运的建模以及这些模型在预测柱色谱在处理条件下的行为方面的应用。 这项研究将提供一个更高层次的了解在这些复杂的介质中的传输现象,以及模型,以帮助设计和优化色谱过程。 这项研究将产生多方面的广泛影响。 它将指导介质制造商开发优化的固定相。 它还将为速率模型的制定提供基础,以预测和优化色谱分离,从而使制药公司能够改进生物工艺的设计和效率。由于分离和纯化通常是生物加工中成本最高的步骤,因此迫切需要经过培训的分离科学家和工程师开发和实施新型分离介质,将广泛的实验和建模方法整合在一起,以提高工业生物分离的有效性。

项目成果

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Giorgio Carta其他文献

Effects of compressive load and support damping on the propagation of flexural waves in beams resting on elastic foundation
  • DOI:
    10.1007/s00419-012-0611-y
  • 发表时间:
    2012-01-24
  • 期刊:
  • 影响因子:
    2.500
  • 作者:
    Giorgio Carta
  • 通讯作者:
    Giorgio Carta
Efficient calculation of the equilibrium composition in multicomponent batch adsorption with the steric mass action model
Adsorption Calculations Using the Film Model Approximation for Intraparticle Mass Transfer
Direct calculation of the equilibrium composition for multi-component Langmuir isotherms in batch adsorption
Editorial Board: Biotechnology Journal 2/2024
编辑委员会:《生物技术杂志》2/2024
  • DOI:
    10.1002/biot.202470022
  • 发表时间:
    2024
  • 期刊:
  • 影响因子:
    4.7
  • 作者:
    H. Alper;M. Antoniewicz;N. Borth;Marc Blondel;N. Budisa;Joaquim M. S. Cabral;Manuel Canovas;Giorgio Carta;Hyung Joon Cha;Jo;Matthew Wook Chang;George Guo;Chen;Wilfred Chen;Wen;Andre Choo;Don A. Cowan;M. DeLisa;Ruth Freitag;Jiaoqi Gao;Hikmet Geckil;R. Grabherr;K. Graumann;Phoenestra GmbH;Switzerland Kundl;Mohd Ali;Hassan;V. Hatzimanikatis;Mingtao Huang;Michael Jewett;J. Keasling;Ali Khademhosseini;Dong;Steffen Klamt;Mattheos Koffas;Ashok Kumar;G. Laible;Kong Peng;Lam;Gyun Min Lee;Luke P. Lee;Xiaokun Li;James Liao;Tiangang Liu;Timothy Lu;Bansi Malhotra;D. Mattanovich;T. Nagamune;Peter Neubauer;Jens B. Nielsen;Lars K. Nielsen;B. Nidetzky;Sean P. Palecek;Hyun Gyu Park;Je;Korea;Tai Hyun Park;Brian F. Pfleger;Nathan D. Price;Mikhail L. Rabinovich;Anurag S. Rathore;F. Riske;Anne Skaja Robinson;Cecilia Roque;A. Schmid;H. Steinkellner;Yongjin J. Zhou;H. Gassen;Dipti Dange;E. Stöger;N. Tavernarakis;J. Woodley;Xiaoxia Xia;Weiwen Zhang;A. Jungbauer;Sang Yup Lee
  • 通讯作者:
    Sang Yup Lee

Giorgio Carta的其他文献

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{{ truncateString('Giorgio Carta', 18)}}的其他基金

International Travel: AIChE-SCEJ Co-sponsored Symposium on Biorecognition and Transport Phenomena in Bioseparation and Bio-nanotechnology in Tokyo, Japan, on March 14-17, 2012
国际旅行:AIChE-SCEJ 于 2012 年 3 月 14-17 日在日本东京联合主办生物分离和生物纳米技术中的生物识别和运输现象研讨会
  • 批准号:
    1152403
  • 财政年份:
    2012
  • 资助金额:
    $ 27.7万
  • 项目类别:
    Standard Grant
Integrated Program for Conformational Effects in Protein Chromatography
蛋白质色谱构象效应综合程序
  • 批准号:
    1134256
  • 财政年份:
    2011
  • 资助金额:
    $ 27.7万
  • 项目类别:
    Standard Grant
Nanoscopic Understanding of Protein Transport and Structure Dynamics in Charged Gels for Protein Chromatography
蛋白质色谱带电凝胶中蛋白质运输和结构动力学的纳米级理解
  • 批准号:
    1032727
  • 财政年份:
    2010
  • 资助金额:
    $ 27.7万
  • 项目类别:
    Standard Grant
Novel Adsorbents and Transport Mechanisms for Enhanced Protein Mass Transfer in Ion Exchange Chromatography
用于增强离子交换色谱中蛋白质传质的新型吸附剂和传输机制
  • 批准号:
    0729857
  • 财政年份:
    2007
  • 资助金额:
    $ 27.7万
  • 项目类别:
    Standard Grant
International Travel: 9th International Conference on Fundamentals of Adsorption, FOA9 in Giardini Naxos, Sicily, Italy on May 20-25, 2007
国际旅行:第九届吸附基础国际会议,FOA9,于 2007 年 5 月 20-25 日在意大利西西里岛贾迪尼·纳克索斯举行
  • 批准号:
    0648823
  • 财政年份:
    2006
  • 资助金额:
    $ 27.7万
  • 项目类别:
    Standard Grant
Fundamentals of Protein Transport in Charged Gels for Chromatography and Membrane Applications
用于色谱和膜应用的带电凝胶中蛋白质运输的基础知识
  • 批准号:
    0079334
  • 财政年份:
    2000
  • 资助金额:
    $ 27.7万
  • 项目类别:
    Standard Grant
Protein Transport and Chromatography with Gel-Composite Ion-Exchangers
使用凝胶复合离子交换剂进行蛋白质运输和色谱分析
  • 批准号:
    9709670
  • 财政年份:
    1997
  • 资助金额:
    $ 27.7万
  • 项目类别:
    Standard Grant
Bioprocess Integration for Enzyme - Catalyzed Syntheses
酶催化合成的生物过程集成
  • 批准号:
    9301447
  • 财政年份:
    1993
  • 资助金额:
    $ 27.7万
  • 项目类别:
    Continuing Grant
A Study of Equilibrium, Mass Transfer and Fixed Bed Dynamic Phenomena in Recovery, Separation and Purification of Amino Acids by Ion Exchange
离子交换回收、分离和纯化氨基酸中的平衡、传质和固定床动态现象的研究
  • 批准号:
    8709011
  • 财政年份:
    1987
  • 资助金额:
    $ 27.7万
  • 项目类别:
    Standard Grant

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