The early infection phase of Ustilago maydis: adaption to the plant environment

玉米黑粉菌感染早期阶段：对植物环境的适应

• 批准号: 116161895
• 负责人: Professor Dr. Gunther Döhlemann
• 金额: --
• 依托单位: Lehrstuhl Terrestrische Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/116161895?language=en
• 关键词: early; infection; phase; Ustilago; maydis

The transcriptome and metabolome analyses conducted during the first funding period have allowed us to identify distinct cellular processes that are altered in maize tissue infected by U. maydis. The key processes “cell death regulation”, “defence response” and “hormone signalling” are hallmarks of this compatible interaction. In the next funding period we wish to elucidate, how these cellular processes control establishment of biotrophy. We will study the role of cell death modulators like Cystatins and the BAX INHIBITOR-1 for cell death suppression in maize and barley during the interaction with U. maydis and U. hordei. The impact of gibberellin and auxin signalling on compatibility will be studied by silencing of distinct key regulators. To facilitate rapid identification of novel compatibility factors, virus based gene silencing (VIGS) and overexpression (VIOE) systems for maize and barley will be optimised / established. In collaborative approaches, proteins involved in the U. maydis/maize interaction will also be analysed regarding their role in the other pathosystems studied within FOR 666. We expect that this close interconnection of projects will enable the identification of novel, general mechanisms of compatibility providing a variety of possible applications both in basic research as well as in applied sciences.

在第一个资助期内进行的转录组和代谢组分析使我们能够确定在受U.玉米粉关键过程“细胞死亡调节”、“防御反应”和“激素信号传导”是这种相容相互作用的标志。在下一个资助期内，我们希望阐明这些细胞过程如何控制生物营养的建立。我们将研究在玉米和大麦与U. maydis和U.霍代。将通过沉默不同的关键调控因子来研究赤霉素和生长素信号传导对相容性的影响。为了便于快速鉴定新的相容性因子，将优化/建立玉米和大麦的基于病毒的基因沉默（VIGS）和过表达（VIOE）系统。在合作的方法，蛋白质参与了美国。还将分析玉米/玉米相互作用在FOR 666研究的其他病理系统中的作用。我们希望，这种密切的项目相互联系，将使新的，一般的兼容性机制的识别提供了各种可能的应用，无论是在基础研究，以及在应用科学。

项目成果

Common motifs in the response of cereal primary metabolism to fungal pathogens are not based on similar transcriptional reprogramming

• DOI: 10.3389/fpls.2011.00039
• 发表时间: 2011-01-01
• 期刊: FRONTIERS IN PLANT SCIENCE
• 影响因子: 5.6
• 作者: Voll, Lars Matthias;Horst, Robin Jonathan;Sonnewald, Uwe
• 通讯作者: Sonnewald, Uwe

Utilizing virus-induced gene silencing for the functional characterization of maize genes during infection with the fungal pathogen Ustilago maydis.

利用病毒诱导的基因沉默来表征真菌病原体玉米黑粉菌感染期间玉米基因的功能特征

• DOI: 10.1007/978-1-62703-278-0_4
• 发表时间: 2012
• 期刊: Methods in molecular biology
• 作者: van der Linde K;Mueller A;Hemetsberger C;Kaschani F;van der Hoorn RAL;Doehlemann G
• 通讯作者: Doehlemann G

Maize Tumors Caused by Ustilago maydis Require Organ-Specific Genes in Host and Pathogen

• DOI: 10.1126/science.1185775
• 发表时间: 2010-04-02
• 期刊: SCIENCE
• 影响因子: 56.9
• 作者: Skibbe, David S.;Doehlemann, Gunther;Walbot, Virginia
• 通讯作者: Walbot, Virginia

A model of Ustilago maydis leaf tumor metabolism

• DOI: 10.4161/psb.5.11.13360
• 发表时间: 2010-01-01
• 期刊: PLANT SIGNALING & BEHAVIOR
• 影响因子: 2.9
• 作者: Horst, Robin J.;Doehlemann, Gunther;Voll, Lars M.
• 通讯作者: Voll, Lars M.