U.S.-U.K. Cooperative Research: Enzyme Mimics in the Gas Phase

美英合作研究：气相酶模拟物

• 批准号: 9722029
• 负责人: David Pratt
• 金额: $ 2.25万
• 依托单位: University of Pittsburgh
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9722029&HistoricalAwards=false
• 关键词: U.S.; U.K.; Cooperative; Research; Enzyme

This three-year award for US-UK cooperative research in physical experimental chemistry involves David W. Pratt of the University of Pittsburgh and W.G. Richards and J.P. Simons at Oxford University. The objective of their research is to study supramolecule assemblies of enzyme catalytic sites and bound substrates, including structure and properties of the supramolecule assemblies and interaction among its components. Enzyme and enzyme substrate mimics, designed to model the behavior of the enzymes, serine and cysteine proteases, will be studied in experiments using laser-based electronic spectroscopy and other techniques. They will also use the enzyme mimics to investigate selective binding of xenon at enzyme catalytic sites, thereby providing insight into its potential as a site specific stain in magnetic resonance imaging. The US investigator and his group bring to this collaboration expertise in characterization of enzyme structures using theoretical techniques. This is complemented by British expertise in enzyme mimic preparation and characterization using ab initio techniques. These studies will provide new information about molecular interactions which are responsible for enzyme function.

这个为期三年的奖项是为了奖励美英在物理实验化学方面的合作研究， 涉及到大卫W.匹兹堡大学的普拉特和W. G.理查兹和J.P. 西蒙斯在牛津大学。 他们的研究目标是研究超分子 酶催化位点和结合底物的组装，包括结构和 超分子组装体的性质及其组分之间的相互作用。 酶和酶底物模拟物，旨在模拟酶的行为， 丝氨酸和半胱氨酸蛋白酶，将在实验中使用基于激光的电子 光谱和其他技术。 他们还将使用酶模拟物来研究 氙在酶催化位点的选择性结合，从而提供了深入了解其 作为磁共振成像中的位点特异性染色剂的潜力。 美国调查员和他的小组为这次合作带来了表征方面的专业知识 酶结构的理论研究。 这是英国的补充 在酶模拟物制备和使用从头算技术表征方面的专业知识。 这些研究将提供有关分子相互作用的新信息， 负责酶的功能。