Gene Trap Transposon Sequencing in Arabidopsis

拟南芥基因陷阱转座子测序

• 批准号: 9723671
• 负责人: Robert Martienssen
• 金额: $ 22.8万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-15 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9723671&HistoricalAwards=false
• 关键词: Gene; Trap; Transposon; Sequencing; Arabidopsis

9723671 Martienssen In a few years, the DNA sequence of the Arabidopsis genome will be known. However, there will be many coding regions for which we do not know a function and the developmental context and location in which they are expressed will not be known. The goal of the project is to generate new lines of Arabidopsis each carrying a gene trap or enhancer trap transposon and to sequence the genomic DNA flanking a number of the trapped insertion sites. This work will form the basis for a larger database which can eventually be merged with the genomic sequences, to provide data on cell-specificity and developmental specificity of unknown genes. The collection will become a powerful resource for fine-mapping and positional cloning as well as for local saturation mutagenesis.

9723671马丁森，再过几年，拟南芥基因组的DNA序列就会揭晓。然而，将会有许多我们不知道其功能的编码区，也不知道它们表达的发育背景和位置。该项目的目标是产生新的拟南芥品系，每个品系携带一个基因陷阱或增强子陷阱转座子，并对一些被捕获的插入位点两侧的基因组DNA进行测序。这项工作将形成一个更大的数据库的基础，该数据库最终可以与基因组序列合并，提供未知基因的细胞特异性和发育特异性的数据。该文库将成为精细作图和定位克隆以及局部饱和突变的强大资源。