Gene Trap Transposon Sequencing in Arabidopsis

再过几年，拟南芥基因组的DNA序列就会知道。然而，我们还不知道许多编码区域的功能，也不知道它们表达的发育背景和位置。该项目的目标是产生新的拟南芥品系，每个品系都携带一个基因陷阱或增强子陷阱转座子，并对一些被困插入位点两侧的基因组DNA进行测序。这项工作将形成一个更大的数据库的基础，最终可以与基因组序列合并，以提供未知基因的细胞特异性和发育特异性的数据。该集合将成为精细制图和定位克隆以及局部饱和诱变的有力资源。

9723671 Martienssen In a few years, the DNA sequence of the Arabidopsis genome will be known. However, there will be many coding regions for which we do not know a function and the developmental context and location in which they are expressed will not be known. The goal of the project is to generate new lines of Arabidopsis each carrying a gene trap or enhancer trap transposon and to sequence the genomic DNA flanking a number of the trapped insertion sites. This work will form the basis for a larger database which can eventually be merged with the genomic sequences, to provide data on cell-specificity and developmental specificity of unknown genes. The collection will become a powerful resource for fine-mapping and positional cloning as well as for local saturation mutagenesis.