A Scanning Electron Microscope for Studies in Developmental Biology

用于发育生物学研究的扫描电子显微镜

• 批准号: 9970149
• 负责人: Robert Martienssen
• 金额: $ 12.97万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-15 至 2001-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9970149&HistoricalAwards=false
• 关键词: Scanning; Electron; Microscope; Studies; Developmental

This award supports the acquisition of a scanning electron microscope (SEM) that operates under conventional high vacuum as well as variable pressure mode. The instrument will by used by at least seven research groups at the Cold Spring Harbor Laboratory to study the developmental genetics of plants and animals. Projects that will use the microscope include the analysis of leaf and meristem development in maize and Arabidopsis; analysis of the Arabidopsis flower and ovule development; the developmental genetics of cell death in the nematode C. elegans; developmental biology of GTPase signaling in Drosophila; and the analysis of morphological changes associated with the development of learning and memory in the Drosophila brain. The SEM is essential for these studies.The Plant Group at Cold Spring Harbor Laboratory is engaged in a systematic search for new mutations in Arabidopsis using gene trap transposon mutagenesis. The SEM will provide a means to rapidly characterize these mutations in terms of morphological changes as well as changes in cell shape or specification. In addition the SEM will be used for training graduate students and postdoctoral fellows, and in the Cold Spring Harbor Laboratory course in Arabidopsis molecular genetics, which has a wide impact on the plant field, and in the DNA Learning Center at CSHL. The ease of use of the variable pressure SEM, compared to conventional SEMs, allows samples to be visualized within minutes of dissection without the need for extensive sample preparation, and the automated settings and the standard operations in 'Microsoft Windows' format means that users can be quickly trained. Therefore the impact of this microscope on research and education at Cold Spring Harbor Laboratory will be substantial.

该奖项支持购买在传统高真空和可变压力模式下运行的扫描电子显微镜（SEM）。 该仪器将被冷泉港实验室的至少七个研究小组用于研究动植物的发育遗传学。 将使用显微镜的项目包括玉米和拟南芥的叶和分生组织发育的分析;拟南芥花和胚珠发育的分析;线虫C.优雅的;果蝇中GT3信号的发育生物学;以及与果蝇大脑中学习和记忆发育相关的形态学变化的分析。 冷泉港实验室的植物组正在利用基因陷阱转座子诱变系统地寻找拟南芥中的新突变。 SEM将提供一种手段，以快速表征这些突变的形态变化以及细胞形状或规格的变化。 此外，SEM将用于培训研究生和博士后研究员，并在冷泉港实验室的拟南芥分子遗传学课程，这对植物领域有广泛的影响，并在CSHL的DNA学习中心。 与传统的SEM相比，可变压力SEM的易用性使样品在解剖后几分钟内就可以可视化，而无需大量的样品制备，并且自动设置和“Microsoft Windows”格式的标准操作意味着用户可以快速培训。 因此，这种显微镜对冷泉港实验室的研究和教育的影响将是巨大的。