Molecular Analysis of a Nuclear Mutation Affecting Chloroplast Development

核基因很可能在很大程度上负责， 叶绿体生物发生与叶发育的协调 高等植物 核突变甚至可以识别这些基因核突变甚至可以识别这些基因 当它们不编码丰富的叶绿体蛋白质时， 已广泛应用于叶绿体发育的分析。 然而，多效性效应，特别是在色素的情况下， 缺陷突变体，往往掩盖了生化病变缺陷突变体，往往掩盖了生化病变 负责大多数的突变表型。 转座子诱变 和克隆提供了另一种方法来分析 这些突变。 本提案的主题是 非光合玉米突变体hcf*106。 这种突变有一个 对类囊体膜组织的显著影响是关键， 叶绿体发育的形态学过程。 HCF*106 膜并不缺乏色素或脂质的生物合成，但 不能积累类囊体蛋白的一个子集。 突变 利用转座子穆尔作为分子“标签”， 和遗传参数提出证明的身份 克隆人 本提案中概述的实验旨在 为了确定hcf*106基因产物的分子功能， 类囊体膜的加工，并应提供重要的 深入了解叶绿体发育的遗传调控。

Nuclear genes are likely to be responsible, in large part,for the coordination of chloroplast biogenesis and leafdevelopment in higher plants. Nuclear mutations can identifysuch genes even when they do not encode abundant chloroplastproteins, and have been widely used in the analysis ofchloroplast development. However, pleiotropic effects,particularly in the case of pigment deficient mutants, tendto obscure the biochemical lesion responsible for most mutantphenotypes. Transposon mutagenesis and cloning provide analternative approach to the analysis of these mutations. Thesubject of this proposal is the non.photosynthetic maizemutant hcf*106. This mutation has a dramatic effect onthylakoid membrane organisation a key, morphological processduring chloroplast development. Hcf*106 membranes are notdeficient in pigment or lipid biosynthesis, but fail toaccumulate a subset of thylakoid proteins. The mutant genehas been cloned using the transposon Mul as a molecular"tag" and genetic arguments are presented demonstrating theidentity of the clone. The experiments outlined in this proposal are designed todetermine the molecular function of the hcf*106 gene productin thylakoid membrane elaboration, and should provideimportant insights into genetic regulation of chloroplastdevelopment.