Molecular Analysis of a Nuclear Mutation Affecting Chloroplast Development

影响叶绿体发育的核突变的分子分析

• 批准号: 8904944
• 负责人: Robert Martienssen
• 金额: $ 30.3万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-15 至 1992-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8904944&HistoricalAwards=false
• 关键词: Molecular; Analysis; Nuclear; Mutation; Affecting

Nuclear genes are likely to be responsible, in large part,for the coordination of chloroplast biogenesis and leafdevelopment in higher plants. Nuclear mutations can identifysuch genes even when they do not encode abundant chloroplastproteins, and have been widely used in the analysis ofchloroplast development. However, pleiotropic effects,particularly in the case of pigment deficient mutants, tendto obscure the biochemical lesion responsible for most mutantphenotypes. Transposon mutagenesis and cloning provide analternative approach to the analysis of these mutations. Thesubject of this proposal is the non.photosynthetic maizemutant hcf*106. This mutation has a dramatic effect onthylakoid membrane organisation a key, morphological processduring chloroplast development. Hcf*106 membranes are notdeficient in pigment or lipid biosynthesis, but fail toaccumulate a subset of thylakoid proteins. The mutant genehas been cloned using the transposon Mul as a molecular"tag" and genetic arguments are presented demonstrating theidentity of the clone. The experiments outlined in this proposal are designed todetermine the molecular function of the hcf*106 gene productin thylakoid membrane elaboration, and should provideimportant insights into genetic regulation of chloroplastdevelopment.

核基因可能在很大程度上负责高等植物叶绿体生物发生和叶子发育的协调。 即使这些基因不编码丰富的叶绿体蛋白，核突变也可以识别这些基因，并已广泛用于叶绿体发育分析。 然而，多效性效应，特别是在色素缺陷突变体的情况下，往往会掩盖导致大多数突变表型的生化损伤。 转座子诱变和克隆为分析这些突变提供了另一种方法。 该提案的主题是非光合玉米突变体 hcf*106。 这种突变对类囊体膜组织（叶绿体发育过程中的关键形态过程）具有显着影响。 Hcf*106 膜在色素或脂质生物合成方面并不缺乏，但无法积累类囊体蛋白的子集。 使用转座子Mul作为分子“标签”克隆了突变基因，并提出了遗传论证来证明克隆的身份。 本提案中概述的实验旨在确定 hcf*106 基因产物在类囊体膜精加工中的分子功能，并且应该为叶绿体发育的遗传调控提供重要的见解。