Systematic Transposon Mutagenesis of the Maize Gene Space

玉米基因空间的系统转座子诱变

• 批准号: 0110143
• 负责人: Robert Martienssen
• 金额: $ 199.08万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0110143&HistoricalAwards=false
• 关键词: Systematic; Transposon; Mutagenesis; Maize; Gene

The maize genome probably contains between 50,000 and 100,000 genes clustered in hypomethylated gene rich regions. We have developed a new method for purifying these regions, and we will explore its use in identifying gene function. This will be accomplished by sampling gene-rich portions of the genome that are chemically distinct from the gene poor regions, and then displaying them on microarrays (DNA chips). These microarrays will then be queried using representations of the DNA corresponding to mutated genes from a large population of maize plants that carries transposable elements (jumping genes). We have previously developed one such population (the Maize Targeted Mutagenesis population) with prior funding from the Plant Genome Research Program. By using computational methods, we will combine the data from the two different resources to create an index of maize gene function, represented by maize seed corresponding to each mutated gene. We anticipate making thousands of entries into this index, allowing maize geneticists to systematically determine the functions of indexed genes.

玉米基因组可能包含5万到10万个聚集在低甲基化基因丰富区域的基因。我们已经开发了一种纯化这些区域的新方法，并将探索其在基因功能鉴定中的应用。这将通过对基因组中富含基因的部分进行取样来完成，这些部分在化学上与基因贫乏的区域不同，然后将它们显示在微阵列（DNA芯片）上。然后，这些微阵列将使用来自大量携带转座因子（跳跃基因）的玉米植物种群的突变基因对应的DNA表示进行查询。我们之前在植物基因组研究计划的资助下开发了一个这样的群体（玉米靶向诱变群体）。通过计算方法，我们将两种不同资源的数据结合起来，创建一个玉米基因功能指数，以每个突变基因对应的玉米种子表示。我们期望在这个索引中加入数千个条目，使玉米遗传学家能够系统地确定索引基因的功能。