Molecular Analysis of a Nucelar Mutation Affecting Chloroplast Development

影响叶绿体发育的核突变的分子分析

• 批准号: 9220774
• 负责人: Robert Martienssen
• 金额: $ 15万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-02-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9220774&HistoricalAwards=false
• 关键词: Molecular; Analysis; Nucelar; Mutation; Affecting

High chlorophyll flourescent (hcf) mutants of higher plants represent a class of nuclear genes required for the development of photosynthetically active chloroplasts. Their products may include nuclear-encoded components of the photosynthetic apparatus, as well as enzymes and regulatory genes required for co-factor biosynthesis and plastid gene expression. Some hcf mutants have pleiotropic effects on membrane protein assembly and thylakoid organization. hcf106 is an example of such a mutation in maize: The Hcf106 gene has been cloned by transposon tagging, and the gene product shown to be an integral chloroplast membrane protein. It has a single transmembrane domain, a potential nucleotide binding site, but no other homology to known proteins. Thylakoid membranes from mutant chloroplasts have lost their characteristic lateral heterogeneity, and fail to accumulate a subset of thylakoid membrane proteins. Analysis of derivative alleles, hypoploids and double mutants will be used to genetically define the hcf106 lesion. Immunolocalization by suborganellar fractionation and immuno- electron microscopy will be used to investigate the possible roles of this protein in thylakoid assembly. %%% Photosynthesis is the basis of life on the planet Earth and a complete understanding of the process as well as chloroplast biogenesis is one of the most important goals of biological research.

高等植物高叶绿素荧光素突变体 代表了一类细胞核基因的发展所需的 光合活性叶绿体。 他们的产品可能包括 光合机构的核编码组件，以及 作为辅因子生物合成所需的酶和调节基因 和质体基因表达。 一些hcf突变体具有多效性 对膜蛋白组装和类囊体组织的影响。 hcf 106是玉米中此类突变的一个例子：Hcf 106基因 已通过转座子标签法克隆， 是一个完整的叶绿体膜蛋白。 它具有单个 跨膜结构域，一个潜在的核苷酸结合位点，但没有 与已知蛋白质同源。 突变体类囊体膜 叶绿体失去了它们特有的横向异质性， 并且不能积累类囊体膜蛋白的子集。 对衍生等位基因、亚倍体和双突变体的分析将 用于遗传学上定义HCF 106损伤。 亚细胞器分离免疫定位和免疫- 电子显微镜将被用来调查可能的作用 这种蛋白质在类囊体组装中的作用。 %%% 光合作用是地球上生命的基础， 完全理解这一过程以及叶绿体 生物起源是生物学最重要的目标之一， research.