Acquisition of Instrumentation for Genomics Research

该提案旨在获得促进普渡大学校园基因组学研究所需的仪器。普渡大学在过去的7年里采取了几项重要举措，为遗传学研究提供了强大的力量。这些措施包括重大实验室空间的建设和改造，在遗传学跨学科研究生培训计划的建立，并聘请了几个遗传学教师的专业知识跨越广泛和令人兴奋的主题范围。这种增长势头现在已经适当地定位普渡大学建立普渡基因组学实验室（PGL）。这样一个实验室，一旦配备高通量基因组学数据生成和分析，将服务于三个重要目标：支持8个主要用户的基因组学研究工作，作为一个学生培训设施，支持NSF赞助的植物遗传学研究生培训工作，并促进合作联系，应用DNA为基础的方法，以植物改良。满足这些目标所需的仪器包括电子放射自显影系统、带分子生物学实验室的自动DNA测序系统、凝胶记录系统、CHEF绘图系统、热循环仪、-80 ℃冷冻设备和植物生长室设施。这一设备一旦到位，将能够在重要的植物和fimgal基因组中启动几个大规模的基因组研究项目，迄今为止，这些基因组尚未得到很好的表征。目前，普渡大学校园内缺乏这些设施都阻碍了这些有价值的研究工作。

This proposal seeks to acquire instrumentation needed to facilitate genomics research on the Purdue University campus. Purdue University has undertaken several important initiatives over the past 7 years that have provided substantial strength to genetics research. These include the construction and renovation of significant laboratory space, establishment of an interdisciplinary graduate training program in genetics, and the hiring of several genetics faculty with expertise spanning a broad and exciting range of topics. This growing momentum has now appropriately positioned Purdue University for the establishment of the Purdue Genomics Laboratory (PGL). Such a laboratory, once equipped for high throughput genomics data generation and analysis, will serve three important objectives: To support genomics research efforts of 8 primary users, to serve as a student training facility in support of a NSF-sponsored graduate training effort in plant genetics, and to facilitate collaborative linkages for the application of DNA-based methodologies to plant improvement. The instrumentation needed to meet these objectives includes an electronic autoradiography system, an automated DNA sequencing system with molecular biology labstation, a gel documentation system, CHEF mapping systems, thermocyclers, -80C freezer equipment, and plant growth chamber facilities. This equipment, once in place, would allow the initiation of several large-scale genomics research projects in important plant and fimgal genomes that, to date, have not been well-characterized. At present, the lack of these facilities on the Purdue campus are all that stand in the way of these valuable research endeavors.