NSF/Alfred P. Sloan Foundation Postdoctoral Research Fellowship in Molecular Evolution for FY 1997

摘要 DBI 9750002 Ichiro Matsumura 这一行动为1997年的NSF/Alfred P. Sloan分子进化博士后研究奖学金提供资金。 这些奖学金支持研究涉及的理论，比较，计算，和/或实验分析的生物模式和过程在分子水平的框架内器官的进化变化和适应。这些研究还包括使用分子数据来解决更广泛的进化问题。 每项研究金资助一项研究和培训计划，该计划将在一个赞助实验室进行。 该奖学金的研究和培训计划题为“酶是否像抗体一样进化？并研究了表面环在底物特异性的适应性进化中的作用。 这项研究测试了酶底物特异性的适应性进化，如抗体成熟，主要发生在表面环的理论。 通过随机突变选定的β-葡萄糖醛酸苷酶表面环并筛选改变特异性的突变，将重现大肠杆菌β-葡萄糖醛酸苷酶和β-半乳糖苷酶之间的进化途径。

Abstract DBI 9750002 Ichiro Matsumura This action funds an NSF/Alfred P. Sloan Postdoctoral Research Fellowship in Molecular Evolution for 1997. These fellowships support studies involving the theoretical, comparative, computational, and/or experimental analyses of biological patterns and processes at the molecular level within the framework of organismic evolutionary change and adaptation. These studies also include the use of molecular data to address broader evolutionary questions. Each fellowship supports a research and training plan to be carried out in a sponsoring laboratory. The research and training plan for this fellowship is entitled "Do enzymes evolve like antibodies?" and investigates the role of surface loops in the adaptive evolution of substrate specificity. This study tests the theory that the adaptive evolution of enzyme substrate specificity, like antibody maturation, occurs largely in surface loops. The evolutionary pathway between the betaglucuronidase and betagalactosidase of Escherichia coli will be recapitulated by randomly mutating selected beta-glucuronidase surface loops and screening for mutations that alter specificity.