SBIR Phase I: Multi Photon Detection (MPD) Enhanced Food Quality Diagnostics

SBIR 第一阶段：多光子检测 (MPD) 增强食品质量诊断

• 批准号: 9761457
• 负责人: Andrzej Drukier
• 金额: $ 9.9万
• 依托单位: BioTraces Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9761457&HistoricalAwards=false
• 关键词: SBIR; Phase; Multi; Photon; Detection

*** 9761457 Drukier This is a Small Business Innovation Research Phase I proposal. The research proposed herein addresses a critical need for high low cost and rapid diagnostics for pathogenic bacterial contaminants associated with food home enteric infections. The main goal of the proposed effort is to exploit the superior sensitivity of BioTraces' Multi Photon Detection (MPDTM) technology to develop an integrated test system for use in process diagnostics and monitoring in the food processing industry. This test system will be based upon the complementary techniques of MPD-enhanced quantitative PCR and MPD-enabled direct detection of DNA. We will utilize MPD's 10,000-fold sensitivity advantage over current instrumentation and BioTraces' experience in developing ultra-sensitivity assay protocols to significantly decrease assay tumaround times and improve specificity by minimizing non-specific biological backgrounds. Our objective is to develop tests which are sensitive to less than 10 pathogenic bacteria per sample with turnaround times of less than one hour. The potential market for very rapid ( 30 minutes), high sensitivity ( 10 CFU/ml) assays for food-bome pathogens are expected to account for about 20% of the total food quality testing market, which is currently estimated to be about $5 billion/year worldwide. More generally, the quantitative PCR and DNA direct detection methods to be developed herein will find significant applications in life science research and clinical diagnostics. ***

*** 9761457 Drukier这是一份小企业创新研究的第一阶段提案。本文提出的研究解决了与食物家庭肠道感染相关的致病菌污染物的高、低成本和快速诊断的迫切需要。该计划的主要目标是利用BioTraces的多光子检测（MPDTM）技术的卓越灵敏度，开发一种用于食品加工行业过程诊断和监测的集成测试系统。该测试系统将基于mpd增强的定量PCR和mpd支持的DNA直接检测的互补技术。我们将利用MPD比现有仪器高10000倍的灵敏度优势，以及BioTraces在开发超灵敏度检测方案方面的经验，通过最大限度地减少非特异性生物背景，显著减少检测周转时间，提高特异性。我们的目标是开发对每个样品对少于10种致病菌敏感的测试，周转时间少于一小时。食品病原体快速（30分钟）、高灵敏度（10 CFU/ml）检测的潜在市场预计将占整个食品质量检测市场的20%左右，目前全球食品质量检测市场估计约为50亿美元/年。更广泛地说，本文开发的定量PCR和DNA直接检测方法将在生命科学研究和临床诊断中找到重要的应用。＊＊＊