SBIR Phase I: Multi Photon Detection (MPD) Enhanced Food Quality Diagnostics

*9761457 Drukier这是小型企业创新研究第一阶段的提案。在此提出的研究满足了对与食物家庭肠道感染相关的致病细菌污染物的高、低成本和快速诊断的迫切需要。拟议工作的主要目标是利用BioTraces的多光子检测(MPDTM)技术的卓越灵敏度，开发一种用于食品加工业过程诊断和监控的集成测试系统。该测试系统将基于MPD增强的定量聚合酶链式反应和MPD支持的DNA直接检测的互补技术。我们将利用MPD的10,000倍于当前仪器的灵敏度优势和BioTraces在开发超灵敏度分析协议方面的经验，通过最大限度地减少非特定生物背景来显著减少分析肿瘤周围时间并提高特异性。我们的目标是开发对每个样本对不到10种致病菌敏感的测试，周转时间不到1小时。食品中致病菌的快速(30分钟)、高灵敏度(10cfu/ml)检测的潜在市场预计将占整个食品质量检测市场的20%左右，目前全球食品质量检测市场的规模估计约为50亿美元/年。更广泛地说，本文将开发的定量PCR和DNA直接检测方法将在生命科学研究和临床诊断中获得重要应用。***

*** 9761457 Drukier This is a Small Business Innovation Research Phase I proposal. The research proposed herein addresses a critical need for high low cost and rapid diagnostics for pathogenic bacterial contaminants associated with food home enteric infections. The main goal of the proposed effort is to exploit the superior sensitivity of BioTraces' Multi Photon Detection (MPDTM) technology to develop an integrated test system for use in process diagnostics and monitoring in the food processing industry. This test system will be based upon the complementary techniques of MPD-enhanced quantitative PCR and MPD-enabled direct detection of DNA. We will utilize MPD's 10,000-fold sensitivity advantage over current instrumentation and BioTraces' experience in developing ultra-sensitivity assay protocols to significantly decrease assay tumaround times and improve specificity by minimizing non-specific biological backgrounds. Our objective is to develop tests which are sensitive to less than 10 pathogenic bacteria per sample with turnaround times of less than one hour. The potential market for very rapid ( 30 minutes), high sensitivity ( 10 CFU/ml) assays for food-bome pathogens are expected to account for about 20% of the total food quality testing market, which is currently estimated to be about $5 billion/year worldwide. More generally, the quantitative PCR and DNA direct detection methods to be developed herein will find significant applications in life science research and clinical diagnostics. ***