SBIR Phase I: Innovative Protein Microarrays

SBIR 第一阶段：创新蛋白质微阵列

• 批准号: 0214854
• 负责人: Andrzej Drukier
• 金额: $ 10万
• 依托单位: BioTraces Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0214854&HistoricalAwards=false
• 关键词: SBIR; Phase; Innovative; Protein; Microarrays

This Small Business Innovation Research (SBIR) Phase I project proposes to apply Multi Photon Detection technique (MPD) to quantify proteins bound to a microarray of antibodies. The first arrays will target cytokines, the important secreted proteins that regulate the immune system. The goals of this project are to create an immunoassay based protein-chip for the concurrent measurment of all the cytokines down to the level of a few zeptomole/ sample, to implement improved P-chip designs using peptide nucleic acid linkers, and to study the levels of cytokines in cell cultures and human samples. By analyzing patterns of protein expression and their post-translational modifications, correlations of functions and/or of disease states with specific protein expression patterns are expected to be established.The commercial applications of this project are in the area of medical diagnostics.

这个小型企业创新研究(SBIR)第一阶段项目建议应用多光子检测技术(MPD)来量化与抗体微阵列结合的蛋白质。第一个阵列将针对细胞因子，这是调节免疫系统的重要分泌蛋白。该项目的目标是创建一种基于免疫分析的蛋白质芯片，用于同时测量所有细胞因子，低至几个齐托莫尔/样本的水平，使用多肽核酸连接物实现改进的P-芯片设计，并研究细胞培养和人体样本中细胞因子的水平。通过分析蛋白质表达模式及其翻译后修饰，有望建立功能和/或疾病状态与特定蛋白质表达模式的关联。该项目在医疗诊断领域的商业应用。