NSF-CGP Fellowship: Dispensing and Metering of Nanoliter Liquid Volumes

9819282Jones该奖项提供资金支持罗切斯特大学电子与计算机工程系Thomas Byron Jones博士与日本京都大学机械工程系Masao Washizu博士进行为期9个月的研究访问。他们的研究目标是开发和测试微电极系统，用于使用介电泳（DEP）力来操纵和控制纳米升大小的液体体积。DEP液体控制为化学和细胞/生物过程的IOTAS（微总量分析系统）提供了增强功能的前景，特别是在可用液体或细胞样品非常小，有毒或生物危险或必须保持严格隔离样品的应用中。长期目标将是可编程系统，其中通过微管注入的液体可重复地分配到纳升液滴中，以便一次一次地通过基板传输，用于激光光学或介电测定、混合或分离等处理。本研究中的实验方法将整合由宿主开发的新的电场驱动液滴传输方案，与DEP液体处理相结合，以测量和分配液体形式的水溶液和化合物。一旦成功演示了电压驱动的液滴点胶，合作者将把电极结构与液滴传输方案集成在一起，以创建一个非常基本的m-TAS设备。最终的目标将是一个电压控制的结构，从储液器（可能是通过电介质虹吸）中抽取一微升体积的液体，然后将其分配到纳米升的液滴中。该项目将是互惠互利的，因为合作者的专业知识互补，这将有助于本研究计划的思想、技能和技术的双向交流。本项目由美国国家科学基金会和日本财团全球伙伴关系中心科学奖学金计划资助

9819282JonesThis award provides funds to support a 9-month research visit by Dr. Thomas Byron Jones, Department of Electrical and Computer Engineering, University of Rochester, for collaboration with Dr. Masao Washizu, Department of Mechanical Engineering, Kyoto University, Japan. The goal of their research is to develop and test microelectrode systems for manipulation and control of nanoliter-sized liquid volumes using dielectrophoretic (DEP) forces. DEP liquid control offers the prospect of enhanced functionality for IOTAS (micro total analysis systems) for chemical and cellular/biological processes, especially in applications where available liquid or cell samples are very small, toxic, or biologically hazardous, or where strict isolation of samples must be maintained. A long-term objective would be programmable systems where liquid injected by a micropipette is dispensed reproducibly into nanoliter droplets for transport one-at-a-time across a substrate for such processing as laser-optical or dielectrometric assay, mixing, or separation.The experimental approach in this research will integrate the new electric field-actuated droplet transport scheme, developed by the host, with DEP liquid handling to meter and dispense aqueous solutions and chemical compounds in liquid form. Once successful in demonstrating voltage-actuated droplet dispensing, the collaborators will integrate the electrode structure with the droplet transport scheme to create a very basic m-TAS device. The ultimate objective will be a voltage-controlled structure that draws a microliter volume of liquid from a reservoir (probably via a dielectric siphon) and then dispenses this into nanoliter droplets. The project will be mutually beneficial because of the complementary expertise of the collaborators which will contribute to the two-way exchanges of ideas, skills, and techniques planned for this research. This project is supported under the Science Fellowship Program between the National Science Foundation and the Japan Foundation Center for Global Partnership.***